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BD Pharmingen™ PE Rat Anti-Mouse CD335 (NKp46)
Clone 29A1.4 (RUO)
Flow cytometric analysis of PE anti-mouse CD335 (NKp46) expression on mouse splenocytes. C57BL/6 and BALB/c mouse spleen cells were stained separately with PE anti-mouse CD335 (NKp46) antibody. After washing, C57BL/6 cells were stained with APC-conjugated anti-mouse NK-1.1 (NKR-P1B and NKR-P1C) antibody (Cat. No.550627; left panel) and BALB/c cells were stained with FITC-conjugated anti-mouse CD49b (DX5) antibody (Cat. No.553857; right panel). Two-color dot plots showing the correlated expression patterns of CD335/NKp46 and either NK-1.1/CD161 (C57BL/6 cells; left panel) or DX5/CD49b (BALB/c cells; right panel) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSRII System.
Flow cytometric analysis of PE anti-mouse CD335 (NKp46) expression on mouse splenocytes. C57BL/6 and BALB/c mouse spleen cells were stained separately with PE anti-mouse CD335 (NKp46) antibody. After washing, C57BL/6 cells were stained with APC-conjugated anti-mouse NK-1.1 (NKR-P1B and NKR-P1C) antibody (Cat. No.550627; left panel) and BALB/c cells were stained with FITC-conjugated anti-mouse CD49b (DX5) antibody (Cat. No.553857; right panel). Two-color dot plots showing the correlated expression patterns of CD335/NKp46 and either NK-1.1/CD161 (C57BL/6 cells; left panel) or DX5/CD49b (BALB/c cells; right panel) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSRII System.
Flow cytometric analysis of PE anti-mouse CD335 (NKp46) expression on mouse splenocytes. C57BL/6 and BALB/c mouse spleen cells were stained separately with PE anti-mouse CD335 (NKp46) antibody. After washing, C57BL/6 cells were stained with APC-conjugated anti-mouse NK-1.1 (NKR-P1B and NKR-P1C) antibody (Cat. No.550627; left panel) and BALB/c cells were stained with FITC-conjugated anti-mouse CD49b (DX5) antibody (Cat. No.553857; right panel). Two-color dot plots showing the correlated expression patterns of CD335/NKp46 and either NK-1.1/CD161 (C57BL/6 cells; left panel) or DX5/CD49b (BALB/c cells; right panel) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSRII System.
Flow cytometric analysis of PE anti-mouse CD335 (NKp46) expression on mouse splenocytes. C57BL/6 and BALB/c mouse spleen cells were stained separately with PE anti-mouse CD335 (NKp46) antibody. After washing, C57BL/6 cells were stained with APC-conjugated anti-mouse NK-1.1 (NKR-P1B and NKR-P1C) antibody (Cat. No.550627; left panel) and BALB/c cells were stained with FITC-conjugated anti-mouse CD49b (DX5) antibody (Cat. No.553857; right panel). Two-color dot plots showing the correlated expression patterns of CD335/NKp46 and either NK-1.1/CD161 (C57BL/6 cells; left panel) or DX5/CD49b (BALB/c cells; right panel) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSRII System.
Product Details
BD Pharmingen™
Ncr1; NK-p46; NKp46; mNKp46; MAR1; mAR-1; mouse activating receptor 1; Ly94
Mouse (QC Testing)
Rat IgG2a, κ
Mouse NKP46 Recombinant Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_1727466
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- An isotype control should be used at the same concentration as the antibody of interest.
Companion Products
APC Mouse Anti-Mouse NK-1.1 RUO
Size
0.1 mg
Cat No.
550627
FITC Rat Anti-Mouse CD49b RUO
Size
0.5 mg
Cat No.
553857
560757 Rev. 1
Antibody Details
29A1.4
The monoclonal antibody 29A1.4 specifically binds to mouse CD335, also known as NKp46. NKp46 is a 46 kDa type I transmembrane glycoprotein that is a member of the natural cytotoxicity receptor (NCR) family and immunoglobulin superfamily. NKp46 is encoded by the Ncr1 gene located on chromosome 7. NKp46 functions as a cytotoxicity triggering receptor and is selectively expressed by immature and mature NK cells in all mouse strains tested. NKp46 is detected on a minute fraction of NK-like T cells (less than 2% of NKp46+ express CD3e) but not on CD1d-restricted NKT cells from C57BL/6 mice. When immobilized on tissue culture plates, the 29A1.4 antibody reportedly stimulates NK cells to produce interferon-gamma and to release their cytoplasmic granule contents. Although the ligands for the NKp46 receptor have not been fully characterized, recent evidence indicates that this receptor plays an important role in the NK cell-mediated recognition and killing of some virus-infected cells and tumor cells. The immunogen used to generate the 29A1.4 clone was mouse NKp46-Fc recombinant protein.
560757 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
496 nm, 566 nm
576 nm
560757 Rev.1
Citations & References
View product citations for antibody "560757" on CiteAb
Development References (4)
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Biassoni R, Pessino A, Bottino C, Pende D, Moretta L, Moretta A. The murine homologue of the human NKp46, a triggering receptor involved in the induction of natural cytotoxicity. Eur J Immunol. 1999; 29(3):1014-1020. (Biology). View Reference
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Gazit R, Gruda R, Elboim M, et al. Lethal influenza infection in the absence of the natural killer cell receptor gene Ncr1. Nat Immunol. 2006; 7(5):517-523. (Biology). View Reference
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Joncker NT, Fernandez NC, Treiner E, Vivier E, Raulet DH. NK cell responsiveness is tuned commensurate with the number of inhibitory receptors for self-MHC class I: the rheostat model. J Immunol. 2009; 182(8):4572-4580. (Clone-specific: Flow cytometry). View Reference
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Walzer T, Blery M, Chaix J, et al. Identification, activation, and selective in vivo ablation of mouse NK cells via NKp46. Proc Natl Acad Sci U S A. 2007; 104(9):3384-3389. (Clone-specific: Activation, Flow cytometry). View Reference
560757 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.