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BD Pharmingen™ PE Rat Anti-Human CD184
Clone 1D9 (RUO)
Detection of CXCR4 expression on human peripheral lymphocytes by PE-conjugated 1D9. Human lysed whole blood were stained with 0.5 µg of PE-conjugated 1D9 and anti-human CD4-FITC (Cat. No. 555346) The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed by using PE-conjugated rat IgG2a (Cat. No.555844) as isotype control. The quadrant markers for the bivariate dot plots were set based on the isotype control.
Detection of CXCR4 expression on human peripheral lymphocytes by PE-conjugated 1D9. Human lysed whole blood were stained with 0.5 µg of PE-conjugated 1D9 and anti-human CD4-FITC (Cat. No. 555346) The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed by using PE-conjugated rat IgG2a (Cat. No.555844) as isotype control. The quadrant markers for the bivariate dot plots were set based on the isotype control.
Product Details
BD Pharmingen™
CXCR4, Fusin
Human (QC Testing)
Rat IgG2a, κ
Human CXCR4 fusion protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_394228
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures
The PE-conjugated 1D9 antibody can be used for the immunofluorescent staining and flow cytometric analyses of human leukocytes and cell lines that express CXCR4 (see figure).
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
551510 Rev. 1
Antibody Details
1D9
The monoclonal antibody 1D9 reacts with the human CD184 which is also known as CXC chemokine receptor, CXCR4. CXCR4 (previously known as fusin, LESTR and HUMSTR), a seven-transmembrane, G-protein-coupled receptor, is the specific receptor for CXC chemokines, SDF-1/CXCL12. CXCR4 was widely expressed by hematopoietic and non-hematopoietic cell types including PMN, monocytes, T cells, B cells, CD34+ progenitor cells, endothelial cells, neurons and astrocytes. The human CXCR4 is used by T-tropic HIV-1 as a co-receptor for viral entry. The human CXCR4 gene has been mapped to chromosome 2q21. The immunogen used to generate 1D9 hybridoma was human CXCR4 fusion protein.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
551510 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
496 nm, 566 nm
576 nm
551510 Rev.1
Citations & References
View product citations for antibody "551510" on CiteAb
Development References (11)
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Bleul CC, Farzan M, Choe H, et al. The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entry. Nature. 1996; 382(6594):829-833. (Biology). View Reference
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Bleul CC, Wu L, Hoxie JA, Springer TA, Mackay CR. The HIV coreceptors CXCR4 and CCR5 are differentially expressed and regulated on human T lymphocytes.. Proc Natl Acad Sci U S A. 1997; 94(5):1925-1930. (Biology). View Reference
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Federsppiel B, Melhado IG, Duncan AM, et al. Molecular cloning of the cDNA and chromosomal localization of the gene for a putative seven-transmembrane segment (7-TMS) receptor isolated from human spleen. Genomics. 1993; 16(3):707-712. (Biology). View Reference
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Feng Y, Broder CC, Kennedy PE, Berger EA. HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor. Science. 1996; 272(5263):872-877. (Biology). View Reference
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Forster R, Kremmer E, Schubel A, et al. Intracellular and surface expression of the HIV-1 coreceptor CXCR4/fusin on various leukocyte subsets: rapid internalization and recycling upon activation. J Immunol. 1998; 160(3):1522-1531. (Immunogen). View Reference
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Gupta SK, Lysko PG, Pillarisetti K, Ohlstein E, Stadel JM. Chemokine receptors in human endothelial cells. Functional expression of CXCR4 and its transcriptional regulation by inflammatory cytokines. J Biol Chem. 1998; 273(7):4282-4287. (Biology). View Reference
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He J, Chen Y, Farzan M, et al. CCR3 and CCR5 are co-receptors for HIV-1 infection of microglia. Nature. 1997; 385(6617):645-649. (Biology). View Reference
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Hesselgesser J, Halks-Miller M, DelVecchio V, et al. CD4-independent association between HIV-1 gp120 and CXCR4: functional chemokine receptors are expressed in human neurons. Curr Biol. 1997; 7(2):112-121. (Biology). View Reference
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Loetscher M, Geiser T, O'Reilly T, Zwahlen R, Baggiolini M, Moser B. Cloning of a human seven-transmembrane domain receptor, LESTR, that is highly expressed in leukocytes. J Biol Chem. 1994; 269(1):232-237. (Biology). View Reference
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Nomura H, Nielsen BW, Matsushima K. Molecular cloning of cDNAs encoding a LD78 receptor and putative leukocyte chemotactic peptide receptors. Int Immunol. 1993; 5(10):1239-1249. (Biology). View Reference
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Oberlin E, Amara A, Bachelerie F, et al. The CXC chemokine SDF-1 is the ligand for LESTR/fusin and prevents infection by T-cell-line-adapted HIV-1. Nature. 1996; 382(6594):833-835. (Biology). View Reference
551510 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.