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FITC Mouse Anti-Human CD73
FITC Mouse Anti-Human CD73

Flow cytometric analysis of CD73 expression on human mesenchymal stem cells. Human mesenchymal stem cells (Lonza) at passage 7 were harvested using Accutase™ Cell Detachment Solution (Cat. No. 561527) and stained with FITC Mouse Anti-Human CD73 antibody (Cat. No. 561254; solid line histogram) or a FITC Mouse IgG1, κ Isotype Control (Cat. No. 554679, dashed line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometry System.

FITC Mouse Anti-Human CD73

Flow cytometric analysis of CD73 on human lymphocytes. Whole blood was stained with FITC Mouse anti-Human CD73 (Cat. No. 561254; solid line fluorescence histogram) and compared with whole blood stained with FITC Mouse IgG1, κ Isotype Control (Cat. No.554679; used at a matching concentration; dashed line histogram). The erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). Flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR™ II Flow Cytometry System.

Flow cytometric analysis of CD73 expression on human mesenchymal stem cells. Human mesenchymal stem cells (Lonza) at passage 7 were harvested using Accutase™ Cell Detachment Solution (Cat. No. 561527) and stained with FITC Mouse Anti-Human CD73 antibody (Cat. No. 561254; solid line histogram) or a FITC Mouse IgG1, κ Isotype Control (Cat. No. 554679, dashed line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometry System.

Flow cytometric analysis of CD73 on human lymphocytes. Whole blood was stained with FITC Mouse anti-Human CD73 (Cat. No. 561254; solid line fluorescence histogram) and compared with whole blood stained with FITC Mouse IgG1, κ Isotype Control (Cat. No.554679; used at a matching concentration; dashed line histogram). The erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). Flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR™ II Flow Cytometry System.

Product Details
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BD Pharmingen™
NT5E; 5' nucleotidase; 5'-NT; E5NT; Ecto-5'-nucleotidase; eN; eNT; NT; NT5
Human (QC Testing)
Mouse IgG1, κ
Pre-B leukemia cell line
Flow cytometry (Routinely Tested)
5 µl
V B-CD73.3
4907
AB_10894209
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
561254 Rev. 1
Antibody Details
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AD2

The AD2 monoclonal antibody specifically binds to ecto-5'-nucleotidase, a 70 kDa, glycosyl phosphatidylinositol (GPI)-anchored glycoprotein. CD73 is expressed on bone marrow derived multipotent mesenchymal stem cells (MSCs), also sometimes identified as mesenchymal stem cells, and is one of the three positive markers that has been identified by the International Society for Cell Terapy (ISCT) for the minimum criteria for identifying MSCs.  Additionally CD73 is expressed on subsets of T and B lymphocytes, follicular dendritic cells, epithelial cells, and endothelial cells. Its expression on lymphocytes increases during T and B cell development. CD73 has enzymatic activity and catalyzes the dephosphorylation of adenosine monophosphate (AMP) converting it to adenosine. It has been suggested that CD73 can mediate costimulatory signals in T cell activation and adhesion of lymphocytes to endothelium.

Due to the expression characteristics of this molecule on different cell types, this FITC format is only recommended for use on cells expressing high amounts of CD73, such as MSCs.

561254 Rev. 1
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
FITC
Blue 488 nm
494 nm
518 nm
561254 Rev.1
Citations & References
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Development References (6)

  1. Airas L, Salmi M, Jalkanen S. Lymphocyte-vascular adhesion protein-2 is a novel 70-kDa molecule involved in lymphocyte adhesion to vascular endothelium. J Immunol. 1993; 151(8):4228-4238. (Biology). View Reference
  2. Alam MS, Kurtz CC, Rowlett RM, et al. CD73 is expressed by human regulatory T helper cells and suppresses proinflammatory cytokine production and Helicobacter felis-induced gastritis in mice. J Infect Dis. 2009; 199(4):494-504. (Biology). View Reference
  3. Dominici M, Le Blanc K, Mueller I, et. al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006; 8(4):315-317. (Biology). View Reference
  4. Salazar-Gonzalez JF, Moody DJ, Giorgi JV, Martinez-Maza O, Mitsuyasu RT, Fahey JL. Reduced ecto-5'-nucleotidase activity and enhanced OKT10 and HLA-DR expression on CD8 (T suppressor/cytotoxic) lymphocytes in the acquired immune deficiency syndrome: evidence of CD8 cell immaturity. J Immunol. 1985; 135(3):1778-1785. (Biology). View Reference
  5. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
  6. Thomson LF, Ruedi JM, Glass A, et al. Production and characterization of monoclonal antibodies to the glycosyl phosphatidylinositol-anchored lymphocyte differentiation antigen ecto-5'-nucleotidase (CD73). Tissue Antigens. 1990; 35(1):9-19. (Biology). View Reference
View All (6) View Less
561254 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.