Dear Customer, If you experience any text readability or display issues on our website, please update your web browser to the latest version
See More
BD Pharmingen™ FITC Mouse Anti-Human CD68
Clone Y1/82A (RUO)
Flow cytometric analysis of CD68 expression on human peripheral blood monocytes. Human peripheral blood mononuclear cells were fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution (Cat. No. 554722). After washing with BD Perm/Wash™ Buffer (Cat. No. 554723), the cells were stained with either FITC Mouse Anti-Human CD68 antibody (Cat. No. 562117; solid line histogram) or with a FITC Mouse IgG2b, κ Isotype Control (Cat. No. 555057; dashed line histogram). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Flow cytometric analysis of CD68 expression on human peripheral blood monocytes. Human peripheral blood mononuclear cells were fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution (Cat. No. 554722). After washing with BD Perm/Wash™ Buffer (Cat. No. 554723), the cells were stained with either FITC Mouse Anti-Human CD68 antibody (Cat. No. 562117; solid line histogram) or with a FITC Mouse IgG2b, κ Isotype Control (Cat. No. 555057; dashed line histogram). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Product Details
BD Pharmingen™
GP110; Macrosialin; SCARD1; Scavenger receptor class D, member 1
Human (QC Testing)
Mouse BALB/c IgG2b, κ
PHA-stimulated Human PBMC
Intracellular staining (flow cytometry) (Routinely Tested)
5 µl
IV M149; VI MR23
AB_10896283
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Fixation and Permeabilization Solution RUO
Size
125 ML
Cat No.
554722
Perm/Wash Buffer RUO
Size
100 ML
Cat No.
554723
FITC Mouse IgG2b, κ Isotype Control RUO
Size
0.1 mg
Cat No.
555057
562117 Rev. 1
Antibody Details
Y1/82A
The Y1/82A monoclonal antibody specifically binds to CD68 which is also known as, Scavenger receptor class D member 1 (SCARD1), Macrosialin, or GP110. CD68 is a cell surface 110 kDa-type I-transmembrane glycoprotein that is primarily expressed in cytoplasmic granules of monocytes, macrophages, dendritic cells, granulocytes, myeloid progenitor cells and, reportedly, a subset of CD34-positive hemopoietic bone marrow progenitor cells. CD68 belongs to the sialomucin family and serves as a scavenger receptor that can bind and internalize oxidized low density lipoproteins (LDL). This antibody is useful in studies of myeloid cell development and function.
562117 Rev. 1
Format Details
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
494 nm
518 nm
562117 Rev.1
Citations & References
View product citations for antibody "562117" on CiteAb
Development References (4)
-
Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
-
Davey FR, Cordell JL, Erber WN, Pulford KA, Gatter KC, Mason DY. Monoclonal antibody (Y1/82A) with specificity towards peripheral blood monocytes and tissue macrophages. J Clin Pathol. 1988; 41(7):753-758. (Clone-specific: Immunohistochemistry). View Reference
-
Davey FR, Erber WN, Gatter KC, Mason DY. The use of monoclonal antibody Y1/82A in the identification of acute myeloblastic and monocytic leukemias. Am J Clin Pathol. 1988; 89(1):76-80. (Clone-specific: Immunohistochemistry). View Reference
-
Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
562117 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.