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BD Horizon™ BV421 Rat Anti-Mouse CD107a
Clone 1D4B (RUO)
Flow cytometric analysis of CD107a expression in mouse splenocytes. Mouse splenic leucocytes were fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722). The cells were washed and subsequently stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; dashed line histogram) or BD Horizon BV421 Rat Anti-Mouse CD107a antibody (Cat. No. 564347; solid line histogram). Fluorescence histograms showing the expression of CD107a (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Flow cytometric analysis of CD107a expression in mouse splenocytes. Mouse splenic leucocytes were fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722). The cells were washed and subsequently stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; dashed line histogram) or BD Horizon BV421 Rat Anti-Mouse CD107a antibody (Cat. No. 564347; solid line histogram). Fluorescence histograms showing the expression of CD107a (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Flow cytometric analysis of CD107a expression in mouse splenocytes. Mouse splenic leucocytes were fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722). The cells were washed and subsequently stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; dashed line histogram) or BD Horizon BV421 Rat Anti-Mouse CD107a antibody (Cat. No. 564347; solid line histogram). Fluorescence histograms showing the expression of CD107a (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Flow cytometric analysis of CD107a expression in mouse splenocytes. Mouse splenic leucocytes were fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722). The cells were washed and subsequently stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; dashed line histogram) or BD Horizon BV421 Rat Anti-Mouse CD107a antibody (Cat. No. 564347; solid line histogram). Fluorescence histograms showing the expression of CD107a (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Product Details
BD Horizon™
LAMP-1; LGP-120; LGP-A; P2B
Mouse (QC Testing)
Rat SD, also known as Sprague-Dawley (outbred) IgG2a, κ
Plasma membrane fraction of mouse embryo NIH 3T3 cell line
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2738760
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
Fixation and Permeabilization Solution RUO
Size
125 ML
Cat No.
554722
Perm/Wash Buffer RUO
Size
100 ML
Cat No.
554723
BV421 Rat IgG2a, κ Isotype Control RUO
Size
50 µg
Cat No.
562602
564347 Rev. 1
Antibody Details
1D4B
The 1D4B antibody recognizes CD107a which is also known as, Lysosome-Associated Membrane Protein 1 (LAMP-1). CD107a is one of the two major glycoproteins in lysosome membranes that provide useful markers to distinguish lysosomes from other organelles. CD107a may play a role in the lysosomal degradation of certain molecules. Mouse CD107a is a type I transmembrane glycoprotein. It consists of a 40-kDa core protein which is heavily glycosylated to form heterogeneous mature glycoprotein of 110-140 kDa. It is principally expressed in epithelial cells and macrophages in a variety of organs. Following activation, CD107a is relocated to the surface of some lymphocytes, macrophages, epithelial cells, endothelial cells, platelets, and tumor cells. Cell-surface CD107a may participate in intercellular adhesion and adhesion to the extracellular matrix. Cell surface CD107a expression can serve as a useful marker for cytotoxic NK and CD8+ T cells, as well as, some malignant tumor cells.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.
564347 Rev. 1
Format Details
BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV421
407 nm
423 nm
564347 Rev.1
Citations & References
View product citations for antibody "564347" on CiteAb
Development References (5)
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Arterburn LM, Earles BJ, August JT. The disulfide structure of mouse lysosome-associated membrane protein 1. J Biol Chem. 1990; 265(13):7419-7423. (Biology). View Reference
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Chen JW, Chen GL, D'Souza MP, Murphy TL, August JT. Lysosomal membrane glycoproteins: properties of LAMP-1 and LAMP-2. Biochem Soc Symp. 1986; 51:97-112. (Biology). View Reference
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Chen JW, Murphy TL, Willingham MC, Pastan I, August JT. Identification of two lysosomal membrane glycoproteins. J Cell Biol. 1985; 101(1):85-95. (Biology). View Reference
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Chen JW, Pan W, D'Souza MP, August JT. Lysosome-associated membrane proteins: characterization of LAMP-1 of macrophage P388 and mouse embryo 3T3 cultured cells. Arch Biochem Biophys. 1985; 239(2):574-586. (Immunogen). View Reference
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Rohrer J, Schweizer A, Russell D, Kornfeld S. The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane. J Cell Biol. 1996; 132(4):565-576. (Biology). View Reference
564347 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.