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BB700 Rat Anti-Mouse CD56 (NCAM-1)
Product Details
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BD OptiBuild™
CD56; E-NCAM; N-CAM-1; Ncam; Ncam1
Mouse (Tested in Development)
Rat IgG2a, κ
Mouse NCAM-1 Recombinant Protein
Flow cytometry (Qualified)
0.2 mg/ml
AB_2872553
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BB700 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Blue 700 is covered by one or more of the following US patents: 8,455,613 and 8,575,303.
  10. Cy is a trademark of GE Healthcare.
748092 Rev. 2
Antibody Details
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809220

The 809220 monoclonal antibody specifically recognizes CD56 which is also known as Neural cell adhesion molecule 1 (NCAM-1), Neural cell adhesion molecule (NCAM), or embryonic NCAM (E-NCAM). CD56 (NCAM-1) is a 120-180 kDa type I transmembrane glycoprotein that is encoded by Ncam1 which belongs to the immunoglobulin superfamily (IgSF). Three different isoforms of CD56 (NCAM-1) have been described that vary in their cytoplasmic domains. The 180 kDa long isoform contains five consecutive IgC-like domains followed by two fibronectin type III domains in its extracellular region, a transmembrane sequence and a large cytoplasmic domain. A 140 kDa isoform has a shorter cytoplasmic tail whereas the 120 kDa isoform is glycophosphatidylinositol (GPI)-linked to the cell membrane. CD56 (NCAM-1) functions on cells as a hemophilic or heterophilic adhesion molecule and as a receptor for various ligands including certain growth factors. Throughout development and adulthood, CD56 (NCAM-1) is expressed on various cell types such as neurons where it might play roles in cellular migration, axonal guidance, and synapse formation. It can also be expressed on astrocytes as well as bone marrow neutrophils and some monocytes and might be involved in cellular migration and other functions. Polysialylation of CD56 (NCAM-1) reduces its ability to dimerize and can thereby affect the functions of cells that express it.

748092 Rev. 2
Format Details
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BB700
The BD Horizon Brilliant™ Blue 700 (BB700) Dye is part of the BD Horizon Brilliant™ Blue family of dyes. This tandem fluorochrome is comprised of a polymer-technology dye donor with an excitation maximum (Ex Max) of 476-nm and an acceptor dye with an emission maximum (Em Max) at 695-nm. Driven by BD innovation, BB700 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 695-nm (e.g., a 695/20-nm bandpass filter). The donor dye can be excited by the Violet (405 nm) laser and the acceptor dye can be excited by the red (627–640 nm) laser resulting in cross-laser excitation and fluorescence spillover. BB700 Reagents are significantly brighter than equivalent PerCP or PerCP-Cy5.5 reagents and are less sensitive to photobleaching. In addition, BB700 shows much less excitation by the violet (407-nm) laser resulting in less spillover. BB700 has minimal yellow green (562-nm) excitation and is ideal for instruments with both blue (488-nm) and yellow green (562-nm) lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BB700
Blue 488 nm
476 nm
695 nm
748092 Rev.2
Citations & References
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View product citations for antibody "748092" on CiteAb

Development References (4)

  1. Fujita T, Chen MJ, Li B, et al. Neuronal transgene expression in dominant-negative SNARE mice.. J Neurosci. 2014; 34(50):16594-604. (Clone-specific: Fluorescence activated cell sorting). View Reference
  2. Li S, Nie EH, Yin Y, et al. GDF10 is a signal for axonal sprouting and functional recovery after stroke.. Nat Neurosci. 2015; 18(12):1737-45. (Clone-specific: Fluorescence activated cell sorting). View Reference
  3. Rougon G, Deagostini-Bazin H, Hirn M, Goridis C. Tissue- and developmental stage-specific forms of a neural cell surface antigen linked to differences in glycosylation of a common polypeptide.. EMBO J. 1982; 1(10):1239-44. (Biology). View Reference
  4. Stamatos NM, Zhang L, Jokilammi A, et al. Changes in polysialic acid expression on myeloid cells during differentiation and recruitment to sites of inflammation: role in phagocytosis. Glycobiology. 2014; 24(9):864-879. (Biology). View Reference
View All (4) View Less
748092 Rev. 2

 

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.