Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
Australia (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Link Arrow
Solutions

Link Arrow
Discover & Learn

Link Arrow
Resources & Tools

Link Arrow
Support

Link Arrow
Search icon Search icon
Close Menu
      Alert Icon
      APC Mouse Anti-Rat CD3
      APC Mouse Anti-Rat CD3
      Two-color analysis of the expression of CD3 on rat splenocytes. A single-cell suspension of Lewis splenocytes was simultaneously incubated with the APC-conjugated 1F4 mAb and FITC-conjugated anti-rat CD5 mAb OX-19 (Cat. No. 551449). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
      APC Mouse Anti-Rat CD3
      Two-color analysis of the expression of CD3 on rat splenocytes. A single-cell suspension of Lewis splenocytes was simultaneously incubated with the APC-conjugated 1F4 mAb and FITC-conjugated anti-rat CD5 mAb OX-19 (Cat. No. 551449). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
      Product Details
      Down Arrow Up Arrow


      BD Pharmingen™
      Rat (QC Testing)
      Mouse BALB/c IgM, κ
      F344 rat splenic T cells stimulated with PMA plus A23187
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      AB_398622
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
      Show More blue down arrow Show Less blue up arrow

      Recommended Assay Procedures

      The APC fluorochrome is excited by laser lines from 595 to 647 nm, and its emission is collected in a detector for fluorescence wavelengths between 640 and 680 nm.

      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      Show More blue down arrow Show Less blue up arrow
      557030 Rev. 5
      Antibody Details
      Down Arrow Up Arrow
      1F4

      The 1F4 antibody reacts with the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes, peripheral T lymphocytes, and dendritic epidermal T cells. In vitro, immobilized 1F4 mAb induces T-cell proliferation, and soluble mAb synergizes with PMA for T-cell activation. Pre-incubation of splenocytes with mAb 1F4 blocks staining with mAb G4.18 (Cat. No. 559974/554830).

      557030 Rev. 5
      Format Details
      Down Arrow Up Arrow
      APC
      Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      APC
      Red 627-640 nm
      651 nm
      660 nm
      557030 Rev.5
      Citations & References
      Down Arrow Up Arrow
      View product citations for antibody "557030" on CiteAb

      Development References (3)

      1. Elbe A, Kilgus O, Hünig T, and Stingl G. T-cell receptor diversity in dendritic epidermal T cells in the rat. J Invest Dermatol. 1993; 102:74-79. (Biology). View Reference
      2. Nicolls MR, Aversa GG, Pearce NW, et al. Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody.. Transplantation. 1993; 55(3):459-68. (Biology). View Reference
      3. Tanaka T, Masuko T, Yagita H, Tamura T, Hashimoto Y. Characterization of a CD3-like rat T cell surface antigen recognized by a monoclonal antibody.. J Immunol. 1989; 142(8):2791-2795. (Immunogen). View Reference
      557030 Rev. 5

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

      Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.