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Alexa Fluor® 647 Mouse Anti-Human CD11c
Alexa Fluor® 647 Mouse Anti-Human CD11c
Two-parameter flow cytometric analysis of CD11c expression on human peripheral blood leucocyte populations. Human peripheral blood  (collected with heparin as the preferred anticoagulant rather than EDTA) was stained with either Alexa Fluor 647 Mouse IgG1, κ Isotype Control (Cat. No. 557714; Left Plot) or  Alexa Fluor 647 Mouse Anti-Human CD11c antibody (Cat. No. 566730; Right Plot) at 0.5 μg. Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter pseudocolor density plots showing the correlated expression of CD11c (or Ig Isotype control staining) versus side light scatter signals (SSC-A) were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD FACSCelesta™ Flow Cytometer System and FlowJo™ software. Data shown in this Technical Data Sheet are not lot specific.
Two-parameter flow cytometric analysis of CD11c expression on human peripheral blood leucocyte populations. Human peripheral blood  (collected with heparin as the preferred anticoagulant rather than EDTA) was stained with either Alexa Fluor 647 Mouse IgG1, κ Isotype Control (Cat. No. 557714; Left Plot) or  Alexa Fluor 647 Mouse Anti-Human CD11c antibody (Cat. No. 566730; Right Plot) at 0.5 μg. Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter pseudocolor density plots showing the correlated expression of CD11c (or Ig Isotype control staining) versus side light scatter signals (SSC-A) were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD FACSCelesta™ Flow Cytometer System and FlowJo™ software. Data shown in this Technical Data Sheet are not lot specific.
Product Details
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BD Pharmingen™
ITGAX; integrin alpha X; CD11C; p150 95 integrin alpha chain; SLEB6
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human Synovial Fluid Dendritic Cells
Flow cytometry (Routinely Tested)
0.2 mg/ml
III 256; V S143
AB_2869834
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Recommended Assay Procedures

Note: The binding of the BU15 antibody to CD11c is divalent cation dependent. Therefore, heparin is recommended for use as the blood anticoagulant rather than the EDTA chelating agent that might adversely affect BU15 antibody binding and cellular staining.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  6. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
  9. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  10. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566731 Rev. 2
Antibody Details
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BU15

The BU15 monoclonal antibody specifically binds to CD11c, which is also known as Integrin alpha X (αX Integrin) or p150,95 Integrin alpha chain. CD11c is a ~150 kDa type I transmembrane glycoprotein that is encoded by ITGAX (Integrin subunit alpha X) which belongs to the integrin alpha chain family. It is variably expressed on monocytes, macrophages, granulocytes, NK cells, dendritic cells, and subsets of B and T cells. CD11c associates with CD18 (Integrin beta 2/β2 Integrin) to form the heterodimeric CD11c:CD18 complex, which is also known as p150,95 Integrin, or the Type 4 Complement Receptor (CR4). CD11c:CD18 binds to fibrinogen, iC3b, ICAM-1 (CD54), or lipopolysaccharide (LPS). CD11c:CD18 functions as an adhesion molecule that mediates cellular binding to ligands expressed on stimulated cells including epithelium and endothelium found during inflammation.

                

566731 Rev. 2
Format Details
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
566731 Rev.2
Citations & References
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Development References (4)

  1. Hogg N, Horton MA. Myeloid antigens: New and previously defined clusters. In: McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:576-602.
  2. Hogg N. Human mononuclear phagocyte molecules and the use of monoclonal antibodies in their detection. Clin Exp Immunol. 1987; 69(3):687-694. (Clone-specific). View Reference
  3. Luk J, Springer TA. CD11c cluster report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:1590-1592.
  4. Zola H, Swart B, Nicholson I, Voss E. CD11c. In: Zola H. Leukocyte and Stromal Cell Molecules : the CD Markers. Hoboken, N.J.: Wiley-Liss; 2007:60.
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566731 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.