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CD4 Intracellular Cytokine Detection Kit Anti-Hu-TNF-α/CD69/CD4/CD3

BD FastImmune™ CD4 Intracellular Cytokine Detection Kit Anti-Hu-TNF-α/CD69/CD4/CD3

Product Details
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BD FastImmune™
Human
Flow cytometry
RUO (GMP)


Description

The BD FastImmune™ CD4 intracellular cytokine detection kit is designed for the detection of intracellular cytokines and the activation marker CD69 in antigen-activated CD4+ T lymphocytes in whole blood. applications include studies of T-cell responses to antigens, such as herpes viruses, HIV, and tumor antigens.

Contents: Antibody Cocktail (TNF-α FITC, CD69 PE, CD4 PerCP-Cy™5.5, CD3 APC), Isotype Ctrl Cocktail, Co-Stimulatory Antibodies (CD28/CD49d), Brefeldin A (BFA) Sol., EDTA Sol., BD FACS™ Lysing Solution (10x), BD FACS Permeabilizing Solution 2 (10x).

Preparation And Storage

Upon receipt, thaw BFA, dispense into 10 µL aliquots, and store at –20°C.

Before use, dilute BD FACS lysing solution and BD FACS Permeabilizing Solution 2 1:10 in deionized (DI) water. Use at room temperature.

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed. Alteration in the appearance of the reagent, such as precipitation or discoloration, indicates instability or deterioration. In such cases, do not use the reagent.

337189 Rev. 1
Components
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Description Quantity/Size Part Number EntrezGene ID
N/A 200.0 N/A N/A
337189 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

 

Although not required, these products are manufactured in accordance with Good Manufacturing Practices.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.