Description
The BD FastImmune™ CD4 intracellular cytokine detection kit is designed for the detection of intracellular cytokines and the activation marker CD69 in antigen-activated CD4+ T lymphocytes in whole blood. applications include studies of T-cell responses to antigens, such as herpes viruses, HIV, and tumor antigens.
Preparation And Storage
Upon receipt, thaw BFA, dispense into 10 µL aliquots, and store at –20°C.
Before use, dilute BD FACS lysing solution and BD FACS Permeabilizing Solution 2 1:10 in deionized (DI) water. Use at room temperature.
Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed. Alteration in the appearance of the reagent, such as precipitation or discoloration, indicates instability or deterioration. In such cases, do not use the reagent.
| Description | Quantity/Size | Part Number | EntrezGene ID |
|---|---|---|---|
| Antibody Cocktail (TNF-α FITC, CD69 PE, CD4 PerCP-Cy™5.5, CD3 APC) | 50 Tests (1 ea) | 337190 | 916,7124,969,920 |
| Anti-Human γ2a FITC/γ1 PE/CD4 PerCP-Cy™5.5/CD3 APC | 50 Tests (1 ea) | 337183 | 916,380793,920 |
| Co-Stimulatory Antibodies (CD28/CD49d) | N/A | 347690 | 940 |
| Brefeldin A (BFA) Solution | N/A | 347688 | N/A |
| EDTA Sol | 2.5 mL (1 ea) | 347689 | N/A |
| BD FACS™ Lysing Solution (10x) | 25 Tests (1 ea) | 347691 | N/A |
| BD FACS Permeabilizing Solution 2 (10x) | 200 Tests (1 ea) | 347692 | N/A |
Development References (15)
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Asanuma H, Sharp M, Maecker HT, Maino VC, Arvin AM. Frequencies of memory T cells specific for varicella-zoster virus, herpes simplex virus, and cytomegalovirus determined by intracellular detection of cytokine expression. J Infec Dis. 2000; 181:859-866. (Biology).
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Clinical Applications of Flow Cytometry: Quality Assurance and Immunophenotyping of Lymphocytes: Approved Guideline. NCCLS document H42-A. 1998. (Biology).
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He XS, Rehermann B, Lopez-Labrador FX, et al. Quantitative analysis of hepatitis C virus-specific CD8+ T cells in peripheral blood and liver using peptide-MHC tetramers. Proc Natl Acad Sci USA. 1999; 96:5692-5697. (Biology).
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Komanduri KV, Viswanathan MN, Wieder ED, et al. Restoration of cytomegalovirus-specific CD4+ T-lymphocyte responses after ganciclovir and highly active antiretroviral therapy in individuals infected with HIV-1. Nat Med. 1998; 4:953-956. (Biology).
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Lee PP, Yee C, Savage PA, et al. Characterization of circulating T cells specific for tumor-associated antigens in melanoma patients. Nat Ned. 1999; 5:677-685. (Biology).
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Maino VC, Picker LJ. Identification of functional subsets by flow cytometry: intracellular detection of cytokine expression.. Cytometry. 1998; 34(5):207-15. (Biology). View Reference
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Maino VC, Suni MA, Wormsley SB, Carlo DJ, Wallace MR, Moss RB. Enhancement of HIV-1 antigen-specific CD4+ T cell memory in subjects with chronic HIV-1 infection receiving an HIV-1 immunogen. AIDS Res Hum Retroviruses. (Biology).
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Maino VC. Rapid assessment of antigen induced cytokine expression in memory T cells by flow cytometry.. Vet Immunol Immunopathol. 1998; 63(1-2):199-207. (Biology). View Reference
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Mehta BA, Maino VC. Simultaneous detection of DNA synthesis and cytokine production in staphylococcal enterotoxin B activated CD4+ T lymphocytes by flow cytometry. J Immunol Methods. 1997; 208:49-59. (Biology).
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Nomura LE, Walker JM, Maecker HT. Optimization of whole blood antigen-specific cytokine assays for CD4+ T cells. Cytometry. 2000; 40:60-68. (Biology).
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Pitcher CJ, Quittner C, Peterson DM, et al. HIV-1-specific CD4+ T cells are detectable in most individuals with active HIV-1 infection, but decline with prolonged viral suppression.. Nat Med. 1999; 5(5):518-25. (Biology). View Reference
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Protection of Laboratory Workers from Infectious Disease Transmitted by Blood, Body Fluids, and Tissue: Tentative Guideline. NCCLS document M29-T2. (Biology).
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Suni MA, Picker LJ, Maino VC. Detection of antigen-specific T cell cytokine expression in whole blood by flow cytometry.. J Immunol Methods. 1998; 212(1):89-98. (Biology). View Reference
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Waldrop S, Pitcher C, Peterson D, Maino V, Picker L. Determination of antigen-specific memory/effector CD4 T cell frequencies by flow cytometry. J Clin Invest. 1997; 99:1739-1750. (Biology).
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Waldrop SL, Davis KA, Maino VC, Picker LJ. Normal human CD4+ memory T cells display broad heterogeneity in their activation threshold for cytokine synthesis.. J Immunol. 1998; 161(10):5284-95. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Although not required, these products are manufactured in accordance with Good Manufacturing Practices.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.