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CD33 BV421
Product Details
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BD Horizon™
Siglec-3; SIGLEC3; Sialic acid-binding Ig-like lectin 3; p67; gp67; My9
Human
Mouse BALB/c IgG1, κ
CD33-transfected FMY9S5 Cells
Flow cytometry
25 μg/mL
5 μL
IV M503
945
ASR


Product Notices

  1. Caution: If you choose to combine two or more BD Horizon Brilliant™ Dyes in the same multicolor staining cocktail, fluorescent dye interactions may cause staining artifacts without the use of a buffering solution, such as the BD Horizon™ Brilliant Stain Buffer.
663980 Rev. 1
Antibody Details
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P67.6

The CD33 antibody, clone P67-6, is derived from the hybridization of Sp2/0 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with FMY9S5 cells containing the CD33 gene.

The CD33 antibody recognizes a human myelomonocytic antigen, with a molecular weight of 67 kilodaltons (kDa).

663980 Rev. 1
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
663980 Rev.1
Citations & References
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663980 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Analyte Specific Reagent. Analytical and performance characteristics are not established.

Analyte Specific Reagent. Analytical and performance characteristics are not established.

 

Documents are subject to revision without notice. Please verify you have the correct revision of the document, and always refer back to BD's eIFU website for the latest and most up to date information.