Description
Becton Dickinson Immunocytometry Systems (BDIS) Simultest™ CD3/CD19 (Leu™-4/12) is a two-color direct immunofluorescence reagent for enumerating percentages of mature human T and B lymphocytes in erythrocyte-lysed whole blood (LWB).
Preparation And Storage
1. For in vitro diagnostic use.
2. When stored at 2° to 8°C, the antibody reagent is stable until the expiration date shown on the label. Do not use after the expiration date.
3. The antibody reagent should not be frozen or exposed to direct light during storage or during incubation with cells. Keep the reagent vial dry.
4. Alteration in the appearance of the reagent, such as precipitation or discoloration, indicates instability or deterioration. In such cases, the reagent should not be used.
5. The antibody reagent contains sodium azide as a preservative; however, care should be taken to avoid microbial contamination, which may cause erroneous results.
| Description | Clone | Isotype | EntrezGene ID |
|---|---|---|---|
| CD3 FITC | SK7 | IgG1, κ | N/A |
| CD19 PE | 4G7 | IgG1, κ | 916 |
Development References (20)
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Dörken B, Möller P, Pezzutto A, Schwartz-Albiez R, Moldenhauer G. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:34-36.
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Haynes BF. Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:3-30.
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Jackson AL, Warner NL. Rose NR, Friedman H, Fahey JL, ed. Manual of Clincial Laboratory Immunology, Third Edition. Washington DC: American Society for Microbiology; 1986:226-235.
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Kan EA, Wang CY, Wang LC, Evans RL. Noncovalently bonded subunits of 22 and 28 kd are rapidly internalized by T cells reacted with anti-Leu-4 antibody. J Immunol. 1983; 131(2):536-539. (Biology). View Reference
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Knowles R. Reinherz E, Haynes B, Nadler L, Bernstein I, ed. Leukocyte Typing II: Human T Lymphocytes. 1986:259-288.
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Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981; 153(2):310-323. (Biology). View Reference
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Nadler LM. B Cell/Leukemia Panel Workshop: summary and comments. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human B Lymphocytes. New York: Springer-Verlag; 1986:3-43.
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National Committee for Clinical Laboratory Standards. Clinical Applications of Flow Cytometry: Quality Assurance and Immunophenotyping of Peripheral Blood Lymphocytes; Tentative Guideline. 1992; 1992. (Biology).
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Ohno T, Kanoh T, Suzuki T, et al. Comparative analysis of lymphocyte phenotypes between carriers of human immunodeficiency virus (HIV) and adult patients with primary immunodeficiency using two-color immunofluorescence flow cytometry.. Tohoku J Exp Med. 1988; 154(2):157-72. (Biology). View Reference
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Prince H, Hirji K, Waldbeser L, Plaeger-Marshall S, Kleinman S, Lanier L. Influence of racial background on the distribution of T cell subsets and Leu-11-positive lymphocytes in healthy blood donors. Diag Immunol. 1985; 3:33-37. (Biology).
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Reichert T, DeBruyere M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol. 1991; 60(2):190-208. (Biology). View Reference
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Schmidt RE. Monoclonal antibodies for diagnosis of immunodeficiencies. Blut. 1989; 59:200-206. (Biology).
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Smolen J, Chused T, Leiserson W, Reeves J, Alling D, Steinberg A. Heterogeneity of immunoregulatory T-cell subsets in systemic lupus erythematosus. Am J Med. 1982; 72:783-790. (Biology).
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van Dongen JJM, Krissansen GW, Wolvers-Tettero ILM, et al. Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood. 1988; 71:603-612. (Biology).
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For In Vitro Diagnostics Use.
Documents are subject to revision without notice. Please verify you have the correct revision of the document, and always refer back to BD's eIFU website for the latest and most up to date information.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.