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Alexa Fluor® 647 Mouse anti-Actin
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This SKU will be discontinuing Apr 2024. For additional support, contact your local applications specialist. Contact Us #
Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG1, κ
Chicken gizzard muscle Actin
Bioimaging (Routinely Tested)
5 µl
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Recommended Assay Procedures

1.        Seed the cells in appropriate culture medium at ~10,000 cells per well in a BD Falcon™ 96-well Imaging Plate (Cat. No. 353219), and

        culture overnight.

2.        Remove the culture medium from the wells, and fix the cells by adding 100 µl of fresh 3.7% Formaldehyde in PBS or BD Cytofix™ fixation buffer (Cat. No. 554655) to each well and incubating for 10 minutes at room temperature (RT).

3.        Remove the fixative from the wells, and permeabilize the cells by adding 100 µl of -20°C 90% methanol or -20°C BD™ Phosflow Perm Buffer III (Cat. No. 558050) to each well and incubating for 5 minutes at RT.

4.        Remove the permeabilizer, and wash the wells twice with 100 μl of 1× PBS.

5.        Remove the PBS, and block the cells by adding 100 µl of blocking buffer (3% FBS in 1× PBS) or BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656) to each well and incubating for 30 minutes at RT.

6.        Remove the blocking buffer, dilute the antibody conjugate 1:10 in blocking buffer or Stain Buffer (FBS), and stain the cells by adding 50 µl of the diluted antibody conjugate to each well and incubating for 1 hour at RT.

7.        Remove the diluted antibody conjugate, and wash the wells three times with 100 μl of 1× PBS.

8.        Remove the PBS, and counter-stain the nuclei by adding 100 μl of a 2 μg/ml solution of Hoechst 33342 (eg, Sigma-Aldrich Cat. No. B2261) in 1× PBS to each well at least 15 minutes before imaging.

9.        View and analyze the cells on an appropriate imaging instrument.

Product Notices

  1. Please refer to for technical protocols.
  2. This reagent has been pre-diluted for use at the recommended Volume per Test when following the Recommended Assay Procedure. A Test is typically ~10,000 cells cultured in a well of a 96-well imaging plate.
  3. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
558624 Rev. 3
Antibody Details
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Changes in cellular morphology, adhesion, and motility occur through the reorganization of the actin cytoskeleton.  This reorganization of actin filaments results from interactions between actin and actin-binding proteins.  Actin is a 42-kDa protein that is known as G-actin in its monomeric form.  Polymerization of G-actin monomers leads to the generation of flexible filaments, 5-9 nm in diameter, called F-actin.  F-actin may be organized in linear bundles called stress fibers or in two-dimensional networks.  The latter are highly concentrated beneath the plasma membrane and form the actin cortex.  Regulation of actin cytoskeletal dynamics occurs through actin-binding proteins.  These proteins bind to G- and/or F-actin and regulate various aspects of actin cytoskeletal dynamics, such as polymerization and depolymerization of actin, cross-linking of actin filaments into bundles, interaction of actin-based structures with membranes and other cytoskeletal elements, and locomotion of actin-based structures.  Thus, the actin cytoskeleton is a complex matrix consisting of G- and F-actin along with the multitude of interactions between these actin forms and a variety of different types of actin-binding proteins.

The C4 monoclonal antibody reacts with all known isoforms of actin in vertebrate muscle and non-muscle cells.  

558624 Rev. 3
Format Details
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
558624 Rev.3
Citations & References
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View product citations for antibody "558624" on CiteAb

Development References (3)

  1. Hanstein C, Lange U, Schneider-Poetsch HA, Grolig F, Wagner G. Detection of actin and localization of phytochrome in the green alga Mougeotia by monocloanl antibodies. Acta Histochem Suppl. 1991; 41:223-230. (Clone-specific). View Reference
  2. Lesssard JL. Two monoclonal antibodies to actin: one muscle selective and one generally reactive. Cell Motil Cytoskeleton. 1988; 10(3):349-362. (Immunogen). View Reference
  3. Pantaloni D, Le Clainche C, Cartier M-F. Mechanism of Actin-Based Motility. Science. 2001; 292:1502-1506. (Biology).
558624 Rev. 3

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.