ELISA: Purified 5A1 anti-mouse CSF-1 antibody (Cat. No. 552513) is useful as a capture antibody for a sandwich ELISA which measures mouse CSF-1 protein levels. Purified 5A1 anti-mouse CSF-1 antibody can be paired with a biotinylated antibody (such as D24) as the detector, with recombinant mouse CSF-1 protein as the standard. This capture antibody should be titrated between 1 -4 µg/ml to determine the optimal coating concentration for ELISA. To obtain linear standard curves, doubling dilutions of recombinant mouse CSF-1 ranging from 2000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit our web site, www.bdbiosciences.com, and go to the protocols section or the chapter on ELISA in the Immune Function Handbook.
Note: This ELISA antibody pair shows no cross-reactivity with the following recombinant human cytokines:
IL-1RI, IL-1RII, IL-1α, IL-1β, IL-2, IL-2sR-α, IL-3, IL-4, IL-5, IL-6, IL-6R gp130, IL-7, IL-8, IL-10, IL-12 p40, IL-12 p70, IL-13, IL-15, TNF, LT-α, sTNFRI, sTNFRII, GM-CSF, TGFβ, IFNγ, CD40L, TRAIL, FasL.
Neutralization: Other reported applications include in vitro neutralization of CSF-1 bioactivity. The no azide/low endotoxin (NA/LE) format of 5A1 (Cat. No. 557858) is recommended for in vitro assays.