Skip to main content Skip to navigation
Hamburger Menu
Search icon
Mini Cart
Empty CartYou are not signed in.
Country Selector Country Selector
India (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Mini Cart
Empty CartYou are not signed in.
Products

Link Arrow
Solutions

Link Arrow
Discover & Learn

Link Arrow
Resources & Tools

Link Arrow
Support

Link Arrow
Search icon Search icon
Close Menu
    Mini Cart
    Empty CartYou are not signed in.
    Expand menu Expand menu
      Loading...
      Alert Icon
      HRP Rat Anti-Mouse IgG2a
      553391
      EA (1 Each)
      1 mL
      Add Product To QuoteAdd to Quote
      Save to Quote ListRequest Bulk Reagent Quote
      Product Details
      Down Arrow


      BD Pharmingen™
      Mouse (QC Testing)
      Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
      Pooled BALB/c and C57BL/6 mouse Ig
      ELISA (Routinely Tested)
      AB_394829
      Aqueous buffered solution containing BSA and ProClin™ 150 as preservative.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with HRP under optimum conditions, and unconjugated antibody and free HRP were removed.
      Show More blue down arrow Show Less blue up arrow

      Recommended Assay Procedures

      ELISA:  For sandwich mouse IgG2a ELISA, HRP-conjugated R19-15 mAb is optimal for detection with purified anti-mouse IgG2a R11-89 mAb (Cat. No. 553446) for capture. Investigators are encouraged to titrate with a suggested starting dilution of 1:1000.  This pair of anti-mouse IgG2a mAbs can effectively quantitate mouse IgG2a of these mouse Igh-C allotypes, in order of sensitivity from highest to lowest: e, a, j, and b.

      Warning:  HRP Rat Anti-Mouse IgG2a contains 0.004% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).

      Hazard statements

      May cause an allergic skin reaction.

      Precautionary statements

      Wear protective gloves / eye protection.

      Wear protective clothing.

      Avoid breathing mist/vapours/spray.

      If skin irritation or rash occurs: Get medical advice/attention.

      IF ON SKIN: Wash with plenty of water.

      Dispose of contents/container in accordance with local/regional/national/international regulations.

      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. ProClin is a trademark of Rohm and Haas Company.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      Show More blue down arrow Show Less blue up arrow

      Data Sheets

      DownloadTechnical Data Sheet (TDS)
      553391 Rev. 18
      Antibody Details
      Down Arrow
      R19-15

      The R19-15 antibody recognizes an epitope in the CH3 domain of mouse IgG2a, with strong reactivity to the Igh-I[a] allotype and weaker reactivity to Igh-I[b]. It does not react with other Ig isotypes. Molecular genetic analyses suggest that the Igh-I[b] allele, which encodes IgG2a[b], is derived from a locus found in several wild mouse subspecies, but not domestic mice, which encodes the IgG2c isotype.

      553391 Rev. 18
      Format Details
      Down Arrow
      HRP
      Horseradish Peroxidase (HRP) is an enzyme that catalyzes the oxidation of various substrates in the presence of hydrogen peroxide resulting in a visible colored product. Antibodies, avidin, streptavidin and other specific binding molecules can be conjugated with HRP and used in applications such as i) ELISA that uses a plate reader to measure absorbance of the colored product, or ii) ELISPOT, Immunohistochemistry (IHC) or Western blot in which a colored product can be measured using light microscopy or image analysis.
      HRP
      553391 Rev.18
      Citations & References
      Down Arrow

      Development References (2)

      1. Martin RM, Silva A, Lew AM. The Igh-1 sequence of the non-obese diabetic (NOD) mouse assigns it to the IgG2c isotype. Immunogenetics. 1997; 46(2):167-168. (Biology). View Reference
      2. Morgado MG, Cam P, Gris-Liebe C, Cazenave PA, Jouvin-Marche E. Further evidence that BALB/c and C57BL/6 gamma 2a genes originate from two distinct isotypes. EMBO J. 1989; 8(11):3245-3251. (Biology). View Reference
      553391 Rev. 18

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.