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HRP Goat Anti-Rabbit IgG
Product Details
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BD Pharmingen™
Rabbit (QC Testing)
ELISA (Routinely Tested), Western blot (Tested During Development)
Aqueous buffered solution containing BSA and ProClin™ 150 as preservative.

Preparation And Storage

Store undiluted at -20°C. Avoid multiple freeze-thaws of product. The polyclonal antibody was purified from antiserum by negative adsorption and affinity chromatography. The antibody was conjugated with HRP under optimum conditions, and unconjugated antibody and free HRP were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. ProClin is a trademark of Rohm and Haas Company.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Please refer to for technical protocols.
554021 Rev. 15
Antibody Details
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Polyclonal HRP Goat Anti-Rabbit IgG is a peroxidase conjugated reagent inteded for use with Rabbit IgG antibodies in the ELISA or western blot applications.   HRP Goat Anti-Rabbit IgG is reported to have minimal crossreactivity with human, mouse or rat serum proteins.  Investigators are highly encouraged to titrate the reagent for specific applications, with a 1:1000 dilution as a suggested starting dilution.  For immunohistochemical staining applications, alternate product Biotin Goat Anti-Rabbit IgG (Cat. No. 550338) is recommended.

Warning:  HRP Goat Anti-Rabbit IgG (Cat# 554021) contains 0.004% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).

Hazard statements

May cause an allergic skin reaction.

Precautionary statements

Wear protective gloves / eye protection.

Wear protective clothing.

Avoid breathing mist/vapours/spray.

If skin irritation or rash occurs: Get medical advice/attention.

IF ON SKIN: Wash with plenty of water.

Dispose of contents/container in accordance with local/regional/national/international regulations.

554021 Rev. 15
Format Details
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Horseradish Peroxidase is an enzyme that is capable of cleaving a substrate into hydrogen peroxide which can then be detected in solution or in situ. Antibodies, avidins, and other specific binding molecules conjugated with horseradish peroxidase, in conjunction with an appropriate substrate system, can be used for multiple ELISA or Immunohistochemical applications.
554021 Rev.15
Citations & References
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554021 Rev. 15

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.