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Anti-Human γ1 PE/CD3 PerCP
Product Details
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BD FastImmune™
Human
Controls
RUO (GMP)
Phosphate buffered saline with BSA and 0.1% sodium azide.


Description

The Mouse γ1 (IgG1) antibody, clone X40, is derived from hybridization of NS-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with KLH.

The CD3 antibody, clone SK7, is derived from hybridization of NS-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes.

The Mouse γ1 (IgG1) antibody recognizes a keyhole limpet hemocyanin (KLH) antigen not expressed on human cells.

The CD3 antibody recognizes the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kilodaltons (kDa). The antigen recognized by the CD3 antibody is non-covalently associated with either α/β or γ/δ TCR (70 to 90 kDa).

Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

340414 Rev. 1
Components
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Description Clone Isotype EntrezGene ID
Mouse IgG1 Isotype Control PE L293 IgG1, κ N/A
CD3 PerCP SK7 IgG1, κ N/A
340414 Rev. 1
Citations & References
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Development References (13)

  1. Brenner M, Groh V, Porcelli A, et al. Knapp W, Dörken B, Gilks W, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. 1989:1049-1053.
  2. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  3. Clevers H, Alarcón B, Wileman T, Terhorst C. The T cell receptor/CD3 complex: a dynamic protein ensemble. Annual Rev Immunol. 1988; 6:629. (Biology).
  4. Clinical and Laboratory Standards Institute. 2005. (Biology).
  5. Evans RL, Wall DW, Platsoucas CD, et al. Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to TH2 antigen. Proc Natl Acad Sci U S A. 1981; 78(1):544-548. (Biology). View Reference
  6. Garson JA, Beverly PCL, Coakham HB, Harper EJ. Monoclonal antibodies against human T lymphocytes label Purkinje neurons of many species. Nature. 1982; 298:375. (Biology).
  7. Haynes B. β2-interferon/B Leukocyte Typing II: Human T Lymphocytes. 1986; 3-30. (Biology).
  8. Kan EA, Wang CY, Wang LC, Evans RL. Noncovalently bonded subunits of 22 and 28 kd are rapidly internalized by T cells reacted with anti-Leu-4 antibody. J Immunol. 1983; 131(2):536-539. (Biology). View Reference
  9. Knowles R. Reinherz E, Haynes B, Nadler L, Bernstein I, ed. Leukocyte Typing II: Human T Lymphocytes. 1986:259-288.
  10. Lanier LL, Allison JP, Phillips JH. Correlation of cell surface antigen expression on human thymocytes by multi-color flow cytometric analysis: implications for differentiation. J Immunol. 1986; 137(8):2501-2507. (Biology). View Reference
  11. Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981; 153(2):310-323. (Biology). View Reference
  12. Maino VC, Suni MA, Ruitenberg JJ. Rapid flow cytometric method for measuring lymphocyte subset activation.. Cytometry. 1995; 20(2):127-33. (Biology). View Reference
  13. van Dongen JJM, Krissansen GW, Wolvers-Tettero ILM, et al. Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood. 1988; 71:603-612. (Biology).
View All (13) View Less
340414 Rev. 1

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