Congratulations! We’ve made it to the end of the Fluorochrome Faceoff games. Throughout this competition, we have highlighted some key fluorochrome characteristics that should be considered when selecting fluorochromes for flow cytometry panels.
As you can see, not all fluorochromes are created equal. Some are bright, some are dim; some are excited by many lasers, some are excited by only one or two; some work well for intracellular staining, some do not; and so on. For example, the StarBright™ Dyes are generally bright with unique spectral profiles but are not available on intracellular markers. The NovaFluor™ Dyes generally have clean spillover profiles but tend to be dimmer and have nonspecific background staining. PE and PerCP tandem dyes have cross laser excitation that limits their utility in higher parameter flow cytometry panels. In comparison, the BD Horizon RealYellow™ and BD Horizon RealBlue™ Dyes generally perform well across all categories measured in this competition. In the chart below, we have summarized the overall performance of the various families of dyes that competed in this Fluorochrome Faceoff.
Fluorochrome Family Summary
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| Dye Family | Spillover | Resolution | Buffer Compatibility | Intracellular Staining | Photostability | Monocyte Background |
|---|---|---|---|---|---|---|
| What Good Looks Like: | 1–2 Peak/s | 3–4 Relative Brightness | Perm III; >50% MFI Compared to Control after Treatment | Catalog Availability | <25% MFI Loss vs Time 0 | No Nonspecific Binding |
| BD Horizon RealYellow™ and RealBlue™ Reagents | ||||||
| StarBright™ Blue and Yellow Reagents | ||||||
| NovaFluor™ Blue and Yellow Reagents | ||||||
| BD Horizon Brilliant™ Blue Reagents | ||||||
| PE and PE Tandems | ||||||
| PerCP and PerCP Tandems |
Note: Additional data supporting the generation of this chart are not shown as part of the Fluorochrome Faceoff games.
Try the Winning Fluorochromes
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Competitors
RB780 | PerCP/Fire™ 780
PE-Cy7 | cFBYG781
Competitors
RB705 | PerCP-Cy5.5
SBB700 | PerCP-eFluor™ 710
BB700 | NFB690
Competitors
RY703 | SBY720
NFY700
Competitors
RB613 | PE-CF594
PE/Dazzle™ 594
Competitors
RY610 | PE-CF594 | SBY605
NF610 | PE-eFluor™ 610
Competitors
RY775 | PE-Cy7 | SBY800
PE/Fire™ 810
BD Horizon RealYellow™ and BD Horizon RealBlue™ Reagents 
Disclaimer: Results and conclusions shown throughout the Fluorochrome Faceoff are based on experiments performed under the conditions described. Users should evaluate reagents with their specific protocols as results may vary with different experimental conditions.
Samples available in participating countries only. Cannot be combined with any other offer. Valid once per user. Offer valid on 25 test/ug size only. There are a limited number of samples available, and BD retains the right to cancel this offer at any time.
BD flow cytometers are Class 1 Laser Products. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
CF is a trademark of Biotium, Inc. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.