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AKP Mouse Anti-Human IgG4
Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG1, κ
ELISA (Routinely Tested)
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with AKP under optimum conditions, and unconjugated antibody and free AKP were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

This antibody can be used to detect human IgG4. For the sandwich human IgG4 ELISA, AKP-conjugated JDC-14 is optimal as detecting MAb with G18-145 (Catalog No. 555784) as capture MAb. Researchers are encouraged to fully titrate each lot to obtain optimal results.  At high concentration, background may be observed on other Ig isotypes.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. Please refer to to access safety data sheets (SDS).
555880 Rev. 9
Antibody Details
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The JDC-14 antibody reacts specifically with human IgG4. It does not react with other Ig isotypes.

555880 Rev. 9
Format Details
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Alkaline phosphatase is an enzyme that is capable of cleaving various substrates, usually producing a detectable product. Antibodies, avidins, and other specific binding molecules conjugated with alkaline phosphatase, in conjunction with an appropriate substrate system, can be used for multiple ELISA or Immunohistochemical applications.
555880 Rev.9
Citations & References
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555880 Rev. 9

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.