Description
The Human Th1/Th2 Cytokine Standard contains a mixture of individually lyophilized recombinant proteins made up of human Interleukin (IL)-2, IL-4, IL-5, IL-6, IL-10, Tumor Necrosis Factor (TNF), and Interferon-γ (IFN-γ). The reconstituted Human Th1/Th2 Cytokine Standard is designed for use as a standard control with the BD™ Cytometric Bead Array (CBA) Human Th1/Th2 Cytokine Kit (Cat. No. 550749 or 551809) and the BD™ Cytometric Bead Array (CBA) Non-Human Primate Th1/Th2 Cytokine Kit (Cat. No. 557800). Please refer to the manual included with these kits for further information.
Preparation And Storage
Lyospheres from 1 vial of the Human Th1/Th2 Cytokine Standard, when reconstituted in 2.0 mL Assay Diluent, approximates the CBA activity of human IL-2, IL-4, IL-5, IL-6, IL-10, TNF, and IFN-γ at a concentration of 5000 pg/mL. Following reconstitution, maintain the Human Th1/Th2 Cytokine Standard at 4°C and use within 12 hours. Any unused reconstituted standard must be discarded after this period (do not store or reuse).
Recommended Assay Procedures
The Human Th1/Th2 Cytokine Standard has been tested with either the BD™ Cytometric Bead Array (CBA) Human Th1/Th2 Cytokine Kit (Cat. No. 550749), the BD™ CBA Human Th1/Th2 Cytokine Kit II (Cat. No. 551809) or the BD™ CBA Non-Human Primate Th1/Th2 Cytokine Kit (Cat. No. 557800) to assure to function as a standard in the CBA assay. Investigators are encouraged to refer to these CBA kit manuals or to the abbreviated instruction below (Preparation of Human Th1/Th2 Cytokine Standard Protocol). The Human Th1/Th2 Cytokine Standard should be reconstituted using Assay Diluent supplied with the aforementioned CBA kits (component no. 51-2432KC) or it may be purchased separately (Cat. No. 560104).
Preparation of Human Th1/Th2 Cytokine Standard: The Human Th1/Th2 Cytokine Standard is lyophilized and should be reconstituted and serially diluted before mixing with CBA capture beads and PE detection reagents.
1. Open one vial of the lyophilized Human Th1/Th2 Cytokine Standard. Transfer all the lyospheres to a polypropylene tube (BD Falcon™, Cat. No. 352097). Label the tube "Top Standard".
2. Reconstitute the standard with 2.0 mL of Assay Diluent. Allow the reconstituted standard to equilibrate for at least 15 minutes before making dilutions. Mix the reconstituted protein by pipette only. Do not vortex or mix vigorously.
3. Label additional 12 x 75 mm tubes (BD Falcon™, Cat. No. 352008) and arrange them in the following order: Top Standard, 1:2, 1:4, 1:8, 1:16, 1:32, 1:64, 1:128, and 1:256.
4. Add 300 µl of Assay Diluent to each of the dilution tubes.
5. Perform a serial dilution by transferring 300 µl from the Top Standard to the 1:2 dilution tube and mix thoroughly. Do not vortex, mix by pipet only. Continue making serial dilutions by transferring 300 µl from the 1:2 tube to the 1:4 tube and so on to the 1:256 tube and mix thoroughly. Prepare one tube containing only Assay Diluent to serve as the 0 pg/mL negative control.
Product Notices
- ProClin is a trademark of Rohm and Haas Company.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- This product is manufactured and sold under license from Pestka Biomedical Laboratories, Inc. (d/b/a PBL InterferonSource) and may be used solely as indicated. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics is strictly prohibited. This product is covered by U.S. Patent No. 5,597,901 and Bulgarian Patent No. BG1895.
- Warning: CBA lyophilized standard contains 0.02% (w/w) and Detection Reagent (Part A) contains 0.002% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May cause an allergic skin reaction.Precautionary statements: Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves/eye/face protection. Wear protective clothing. Avoid breathing mist/vapours/spray. If skin irritation or rash occurs: Get medical advice/attention. IF ON SKIN: Wash with plenty of water. Take off contaminated clothing and wash it before reuse. Dispose of contents/container in accordance with local/regional/national/international regulations.
Companion Products
Development References (2)
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Cook EB, Stahl JL, Lowe L, et al. Simultaneous measurement of six cytokines in a single sample of human tears using microparticle-based flow cytometry: allergics vs. non-allergics. J Immunol Methods. 2001; 254(1-2):109-118. (Biology). View Reference
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Dotti G, Savoldo B, Takahashi S, et al. Adenovector-induced expression of human-CD40-ligand (hCD40L) by multiple myeloma cells. A model for immunotherapy. Exp Hematol. 2001; 29(8):952-961. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
