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Human Th1/Th2 Cytokine Cytometric Bead Array (CBA) Kit

Human Th1/Th2 Cytokine Cytometric Bead Array (CBA) Kit

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BD™ Cytometric Bead Array (CBA)


The BD™ CBA Human Th1/Th2 Cytokine Kit (Catalog No. 550749) can be used to quantitatively measure Interleukin-2 (IL-2), Interleukin-4 (IL-4), Interleukin-5 (IL-5), Interleukin-10 (IL-10), Tumor Necrosis Factor (TNF), and Interferon-γ (IFN-γ) protein levels in a single sample. The kit performance has been optimized for analysis of specific cytokines in tissue culture supernatants, EDTA plasma, and serum samples. The kit provides sufficient reagents for the quantitative analysis of 80 samples.

 Vial label Reagent Quantity
 A1 Human IL-2 Capture Beads 1 vial, 0.8 mL
 A2 Human IL-4 Capture Beads 1 vial, 0.8 mL
 A3 Human IL-5 Capture Beads 1 vial, 0.8 mL
 A4 Human IL-10 Capture Beads 1 vial, 0.8 mL
 A5 Human TNF Capture Beads 1 vial, 0.8 mL
 A6 Human IFN-γ Capture Beads 1 vial, 0.8 mL
 B Human Th1/Th2 PE Detection Reagent 1 vial, 4 mL
 C Human Th1/Th2 Cytokine Standards 2 vials, 0.2 mL lyophilized
 D Cytometer Setup Beads 1 vial, 1.5 mL
 E1 PE Positive Control Detector 1 vial, 0.5 mL
 E2 FITC Positive Control Detector 1 vial, 0.5 mL
 F Wash Buffer 1 bottle, 130 mL
 G Assay Diluent 1 bottle, 30 mL
 H Serum Enhancement Buffer 1 bottle, 10 mL


550749 Rev. 1
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Description Quantity/Size Part Number
C) Human Th1/Th2 Cytokine Standards N/A 51-2428KC
E1) PE Positive Control Detector N/A 51-2429KC
B Human Th1/Th2 PE Detection Reagent N/A 51-2427KC
Human IFN-γ Capture Beads 1 Each (1 ea) 51-9005727
Human TNF Capture Beads 1 Each (1 ea) 51-9005724
Human IL-10 Capture Beads 80 Tests (1 ea) 51-9005740
Human IL-5 Capture Beads 1 Each (1 ea) 51-9005718
Human IL-4 Capture Beads 1 Each (1 ea) 51-9005715
Human IL-2 Capture Beads 80 Tests (1 ea) 51-9005736
D Cytometer Setup Beads 1 Each (1 ea) 51-9005731
BD(tm) CBA Serum Enhancement Buffer (H) N/A 51-9002150
G Assay Diluent 1 Each (1 ea) 51-2432KC
F Wash Buffer 1 Each (1 ea) 51-2431KC
E2 FITC Positive Control Detector 1 Each (1 ea) 51-9005734
E1 PE Positive Control Detector 1 Each (1 ea) 51-9005732
550749 Rev. 1
Citations & References
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Development References (13)

  1. Bishop JE, Davis KA. A flow cytometric immunoassay for β2-microglobulin in whole blood. J Immunol Methods. 1997; 210:79–87. (Biology).
  2. Camilla C, Defoort JP, Delaage M, et al. A new flow cytometry-based multi-assay system 1, Application to cytokine immunoassays. Cytometry Suppl. 1998; 8:132. (Biology).
  3. Carson R, Vignali D. Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay. J Immunol Methods. 1999; 227:41–52. (Biology).
  4. Chen, R, L Lowe, et al. Simultaneous quantification of six human cytokines in a single sample using microparticle-based flow cytometric technology. Clin Chem. 1999; 9:1693-1694. (Biology).
  5. Collins DP, Luebering BJ, Shaut DM. T-lymphocyte functionality assessed by analysis of cytokine receptor expression, intracellular cytokine expression, and femtomolar detection of cytokine secretion by quantitative flow cytometry. Cytometry. 1998; 33:249-255. (Biology).
  6. Cook, EB, JL Stahl, et al. Simultaneous measurement of six cytokines in a single sample of human tears using microparticle-based flow cytometry: allergics vs non-allergics. J Immunol Methods. 2001; 254:109-118. (Biology).
  7. Dotti, G, B Salvodo, et al. Adenovector-induced expression of human-CD40-ligand (hCD40L) by multiple myeloma cells: A model for immunotherapy. Exp Hematol. 2001; 29:952-961. (Biology).
  8. Fulton RJ, McDade RL, Smith PL, Kienker LJ, Kettman JR Jr. Advanced multiplexed analysis with the FlowMetrix system. Clin Chem. 1997; 43:1749-1756. (Biology).
  9. Kricka LJ. Diamandis EP, Christopoulos TK, ed. Immunoassay. Academic Press; 1996:389-404.
  10. Lund-Johansen F, Davis K, Bishop J, de Waal Malefyt R. Flow cytometric analysis of immunoprecipitates: high-throughput analysis of protein phosphorylation and protein-protein interactions. Cytometry. 2000; 39(4):250-259. (Biology). View Reference
  11. McHugh TM. Flow microsphere immunoassay for the quantitative and simultaneous detection of multiple soluble analytes. Methods Cell Biol. 1994; 33:613-629. (Biology). View Reference
  12. Oliver KG, Kettman JR, Fulton RJ. Multiplexed analysis of human cytokines by use of the FlowMetrix system. Clin Chem. 1998; 44:2057-2060. (Biology).
  13. Stall, A, Q Sun, et al. A single tube flow cytometric multibead assay for isotyping mouse monoclonal antibodies, Abstract LB77. Experimental Biology Meeting. 1998. (Biology).
View All (13) View Less
550749 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.