Unlock the full potential of your chimeric antigen receptor (CAR) research with our next-generation detection reagent—engineered for unmatched brightness, ease of use and reproducibility.
One-Step Simplicity, No Compromise
Eliminate complex staining protocols. The BD® CAR Detection Reagent is a single-step, ready-to-use solution that streamlines your workflow without sacrificing brightness. Whether you're running a few samples or scaling up, our reagent integrates seamlessly into your flow cytometry assays.
Unparalleled Performance
When it comes to detecting CAR-T cells—especially those with low expression—small differences in performance make a big impact on results. The BD® CAR Detection Reagent consistently delivers exceptional performance across key metrics that matter most to researchers.
Superior Sensitivity
The BD® CAR Detection Reagent reliably detects rare CAR+ cells at frequencies as low as 0.1%, making it ideal for persistence studies and minimal residual disease models. In spiking experiments, our PE-conjugated reagent accurately identified CAR+ cells across a 2-fold dilution series, maintaining linearity and precision even at the lowest concentrations.
Rare Cell Detection. Jurkat-CAR clone isolated from BCMA (scFv)CAR lentivirus-infected cells was spiked into PBMC from a healthy donor in the Associate Sample Collection Program (ASCP) at approximately 4% and 9 additional descending 2-fold dilutions. Jurkat-CAR-spiked PBMC were stained with PE conjugated BD BCMA CAR Detection Reagent at 0.1 μg/test for 30 min at RT, following by flow cytometric analysis. Red box highlights detection of CAR+ cells at ≤0.1% of PBMC.
Brighter Signal, Better Resolution
Compared to currently available reagent options, our reagents demonstrated:
Higher stain index across all fluorochromes (PE, AF488, AF647, BV421)
Clearer separation of CAR+ and CAR– populations
Better resolution of low-affinity CAR constructs
In head-to-head comparisons, BD reagents consistently outperformed others in both signal intensity and background suppression, ensuring confidence in results.
Stain index, normalized to BD® BCMA CAR Detection Reagent, of Jurkat cells infected with BCMA-(single chain variable fragment) CAR and selected for high, medium and low CAR expression.
Robust Across Buffers and Workflows
The BD® CAR Detection Reagents are validated for use with:
Whole blood lysis buffers ( Pharm Lyse™- 555899, FACS™ Lysing Solution - 349202)
Intracellular staining protocols (Perm I - 557885, Transcription Factor Buffer - 562574)
Multi-color panels with minimal spectral spillover
This broad compatibility ensures seamless integration into your existing flow cytometry workflows—without compromising performance.
Download our poster for full performance data and gating strategies.
Extensive Validation
The BD® CAR Detection Reagent has been tested across a range of CAR constructs and expression levels. Results show:
Clear separation of positive and negative populations
Minimal background in PBMCs and whole blood
Able to resolve low frequency CAR populations
Robust performance across instruments
Powered by Our Bright Dyes
Harness the power of our proprietary fluorochromes—exceptionally bright, photostable, and optimized for flow cytometry and backed by the deep dye experience of BD Biosciences. Our conjugates deliver:
Superior stain index
High signal-to-noise ratio
Consistent results across batches
Custom Solutions Available
Need a different fluorochrome or bulk reagents? We offer:
Custom conjugation services
GMP manufacturing compliant with ISO13485
Bulk options
Technical support and panel design from BD scientists
For Research Use Only. Not for use in diagnostic or therapeutic procedures.