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Analysis of Myosin Heavy Chain in human embryonic stem cell (hESC)-derived cardiomyocytes. hESC- derived cardiomyocytes (Evans laboratory, UC San Diego) were disassociated and fixed in BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with BD Phosflow™ Perm/Wash Buffer I (Cat. No. 557885), and stained with PE Mouse Anti-Myosin Heavy Chain (MHC) and Alexa Fluor® 647 Mouse Anti-Cardiac Troponin I (cTnI) (Cat. No. 554409). The cells were first gated on light scatter properties and then analyzed for MHC and cTnI expression. Flow cytometry was performed on a BD LSRFortessa™ flow cytometry system.

Analysis of Myosin Heavy Chain in differentiated C2C12 mouse myoblast cell line. C2C12 cells (ATCC: CRL-1772) that were differentiated for 3 days and then disassociated and fixed in BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with BD Phosflow™ Perm/Wash buffer I (Cat. No. 557885), and stained with PE Mouse Anti-Myosin Heavy Chain (MHC). The cells were first gated on light scatter properties and then analyzed for MHC expression. Flow cytometry was performed on a BD LSRFortessa™ flow cytometry system.
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BD Pharmingen™ PE Mouse Anti-Myosin Heavy Chain

BD Pharmingen™ PE Mouse Anti-Myosin Heavy Chain
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Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
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Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
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The MF20 monoclonal antibody specifically binds to the heavy chain component of skeletal muscle myosin or myosin II. Skeletal muscle myosin is composed of 2 heavy chains and 4 light chains. Myosin Heavy chain is a 220-kDa protein that interacts with actin in the ATP-dependent mechanism of muscle contraction. This antibody is reported to cross react with human, mouse, rat, chicken, dog, frog, and zebrafish.

Development References (5)
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Bader D, Masaki T, Fischman DA. Immunochemical analysis of myosin heavy chain during avian myogenesis in vivo and in vitro. J Cell Biol. 1982; 95(3):763-770. (Immunogen). View Reference
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Dambrot C, Passier R, Atsma D, Mummery CL. Cardiomyocyte differentiation of pluripotent stem cells and their use as cardiac disease models. Biochem J. 2011; 434(1):25-35. (Biology). View Reference
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Lian X, Hsiao C, Wilson G, et al. Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc Natl Acad Sci U S A. 2012; 109(27):E1848-E1857. (Biology). View Reference
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López JE, Myagmar BE, Swigart PM, et al. β-myosin heavy chain is induced by pressure overload in a minor subpopulation of smaller mouse cardiac myocytes. Circ Res. 2011; 109(6):629-638. (Clone-specific). View Reference
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Travaglione S, Messina G, Fabbri A. Cytotoxic necrotizing factor 1 hinders skeletal muscle differentiation in vitro by perturbing the activation/deactivation balance of Rho GTPases. Cell Death Differ. 2005; 12(1):78-86. (Clone-specific). View Reference
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