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Product Details
Product Details
Description
BD Phosflow™ Perm/Wash Buffer I is intended to be used for the intracellular staining of post-translationally modified signaling proteins. BD Phosflow™ Perm/Wash Buffer I is used to permeabilize cells and to serve as an antibody diluent and cell wash buffer. It is optimized for use with the BD Phosflow™ brand of intracellular phosphorylated signaling protein-specific antibodies. BD Phosflow™ Perm/Wash Buffer I is provided as a 10X concentrated solution containing FBS and saponin. The presence of a small amount of precipitate may be observable and will not affect the performance of the buffer. Because saponin-mediated cell permeabilization is a reversible process, it is important to keep the cells in the presence of saponin during intracellular staining.
Preparation And Storage
Store undiluted at 4°C.
Do not freeze.
Recommended Assay Procedures
Flow cytometry: Dilute BD Phosflow™ Perm/Wash Buffer I 1:10 in distilled water prior to use. If desired, the diluted 1X BD™ Phosflow Perm/Wash Buffer I can be passed through a 0.45 µm filter to remove any residual precipitate. The BD Phosflow™ Perm/Wash Buffer I can be used to permeabilize or wash cells and to dilute antibodies for immunofluorescent staining of intracellular proteins.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.