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PE Mouse Anti-Human CXCL16
PE Mouse Anti-Human CXCL16
Flow cytometric analysis of CXCL16 expression on human peripheral blood monocytes. Human whole blood was stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human CXCL16 antibody (Cat. No. 566862; solid line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). A histogram showing the expression of CXCL16 (or Ig Isotype control staining) was derived from gated events with the forward and side-light scatter characteristics of viable monocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
Flow cytometric analysis of CXCL16 expression on human peripheral blood monocytes. Human whole blood was stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human CXCL16 antibody (Cat. No. 566862; solid line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). A histogram showing the expression of CXCL16 (or Ig Isotype control staining) was derived from gated events with the forward and side-light scatter characteristics of viable monocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
Product Details
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BD Pharmingen™
CXCL16; SR-PSOX; SRPSOX; SCYB16
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human SR-PSOX extracellular domain Recombinant Protein
Flow cytometry (Routinely Tested)
5 µl
AB_2869916
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566862 Rev. 1
Antibody Details
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22-19-12

The 22-19-12 monoclonal antibody specifically recognizes CXC chemokine ligand 16 (CXCL16) which is also known as C-X-C motif chemokine 16, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein (SR-PSOX), or Small-inducible cytokine B16 (SCYB16). CXCL16 is a ~30 kDa single-pass type I transmembrane glycoprotein with an extracellular CXC chemokine domain and mucin-like spacer region, followed by a transmembrane region, and a cytoplasmic domain with consensus tyrosine phosphorylation and SH2 binding sites. Transmembrane CXCL16 can serve as a scavenger receptor that binds to oxidized low density lipoprotein, phosphatidylserine, or bacteria and mediates their uptake by macrophages and dendritic cells. Cell surface CXCL16 can be shed in a soluble form after proteolytic cleavage to exert its chemotactic function. CXCL16 is differentially expressed by keratinocytes, monocytes, dendritic cells, endothelial cells, and some activated T cells. CXCL16 exerts its chemotactic function by binding to and signaling through the CXCR6 (CD186) chemokine receptor that is variably expressed by effector/memory CD8+ T cells, CD4+ T cells with Th1- or Th17-like phenotypes, γδ T cells, natural killer (NK) cells, natural killer T (NKT) cells, and monocytes. The 22-19-12 antibody reportedly binds to the chemokine domain of CXCL16 and can inhibit the chemotactic activity of soluble CXCL16.

        

        

566862 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566862 Rev.1
Citations & References
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View product citations for antibody "566862" on CiteAb

Development References (4)

  1. Bachelerie F, Ben-Baruch A, Burkhardt AM, et al. International Union of Basic and Clinical Pharmacology. [corrected]. LXXXIX. Update on the extended family of chemokine receptors and introducing a new nomenclature for atypical chemokine receptors.. Pharmacol Rev. 2014; 66(1):1-79. (Biology). View Reference
  2. Shimaoka T, Kume N, Minami M, et al. Molecular cloning of a novel scavenger receptor for oxidized low density lipoprotein, SR-PSOX, on macrophages. J Biol Chem. 2000; 275(52):40663-40666. (Biology). View Reference
  3. Shimaoka T, Nakayama T, Kume N, et al. Cutting edge: SR-PSOX/CXC chemokine ligand 16 mediates bacterial phagocytosis by APCs through its chemokine domain. J Immunol. 2003; 171(4):1647-1651. (Immunogen: Flow cytometry, Functional assay, Inhibition). View Reference
  4. Tabata S, Kadowaki N, Kitawaki T, et al. Distribution and kinetics of SR-PSOX/CXCL16 and CXCR6 expression on human dendritic cell subsets and CD4+ T cells. J Leukoc Biol. 2005; 77(5):777-786. (Clone-specific: Flow cytometry). View Reference
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566862 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.