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BV786 Mouse Anti-Mouse Ly-108
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Product Details
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BD OptiBuild™
CD352; Slamf6; SLAM family member 6; KAL1; NTB-A; SF2000
Mouse (Tested in Development)
Mouse IgG2a, κ
WT thymocytes
Flow cytometry (Qualified)
0.2 mg/ml
30925
AB_2740648
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV786 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Violet 786 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
  10. Cy is a trademark of Amersham Biosciences Limited.
741030 Rev. 1
Antibody Details
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13G3

The 13G3 monoclonal antibody specifically binds to Lymphocyte antigen 108, Ly-108. Ly-108 is a member of the signaling lymphocytic activation molecule (SLAM) family of immune receptors. Ly-108 is a type 1 transmembrane glycoprotein adhesion receptor that is expressed by T cells, NKT cells, B cells, NK cells, macrophages, dendritic cells and neutrophils. Ly-108 plays multiple roles in innate and adaptive immunity including costimulation of NK cell cytotoxicity and T cell cytokine responses. Moreover, Ly-108 has been implicated in autoimmunity.

The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm.  BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60-nm filter).

741030 Rev. 1
Format Details
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BV786
The BD Horizon Brilliant Violet™ 786 (BV786) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an Ex Max of 407-nm and an acceptor dye with an Em Max at 786-nm.  BV786, driven by BD innovation, is designed to be excited by the violet laser and detected using a filter, centered near 785 nm (e.g. 780/60 nm bandpass filter).  Please ensure that your instrument’s configurations (lasers and filters) are appropriate for this dye.
altImg
BV786
Violet 405 nm
407 nm
786 nm
741030 Rev.1
Citations & References
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View product citations for antibody "741030" on CiteAb

Development References (5)

  1. Griewank K, C Borowski, Rietdijk S, et al. Homotypic interactions mediated by Slamf1 and Slamf6 receptors control NKT cell lineage development. Immunity. 2007; 27(5):751-762. (Clone-specific: Flow cytometry, Immunofluorescence). View Reference
  2. Howie D, Laroux FS, Morra M, et al. Cutting edge: the SLAM family receptor Ly108 controls T cell and neutrophil functions. J Immunol. 2005; 10(174):5931-5935. (Biology). View Reference
  3. Keszei M, Detre C, Rietdijk ST, et al. A novel isoform of the Ly108 gene ameliorates murine lupus.. J Exp Med. 2011; 208(4):811-22. (Immunogen: ELISA, Flow cytometry, Immunoprecipitation). View Reference
  4. Li W, Sofi MH, Rietdijk S, Wang N, et al. The SLAM-Associated Protein (SAP)/Fyn/PKCθ Pathway is Required for Thymocyte-mediated CD4 T Cell Development. Immunity. 2007; 27(2):763-774. (Biology). View Reference
  5. Peck SR, Ruley HE. Ly108: a new member of the mouse CD2 family of cell surface proteins. J Immunol. 2000; 52(1-2):63-72. (Biology). View Reference
View All (5) View Less
741030 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.