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Immunohistochemistry / Immunofluorescence Protocols

Sample Preparation



Immunohistochemistry/Tissue Section Staining

Find protocols for preparing tissue sections for immunostaining manually and using antigen retrieval method. Get the steps for counterstaining and visualization, and visualization using light microscopy..

Immunohistochemical Staining Method for Phospho-proteins in Paraffin Sections

Get step-by-step protocols for deparaffinization and rehydration of tissue slides and for antigen retrieval.

Preparation and Staining of Frozen Tissue Sections

Find protocols for tissue sectioning and immunohistochemical staining of frozen sections.

Preparation and Staining of Paraffin Sections

View protocols for tissue fixation with formalin or zinc and for preparing paraffin sections for immunostaining.



Immunofluorescence Protocols

Cell Staining for Immunofluorescence Microscopy

Get the complete protocol – from fixing to blocking and incubation to preparation for microscopic visualization of cells – for immunofluorescence detection.

Immunofluorescence Staining of Human Cells by Lysed Whole Blood Method

Find a step-by-step protocol for staining human cells by lysed whole blood method for immunofluorescence detection.

Direct Immunofluorescence Staining of Mononuclear Cells

Get a complete protocol for direct immunofluorescence staining of mononuclear cells.