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BD Transduction Laboratories™ Purified Mouse Anti-Dynamin
Clone 41/Dynamin (RUO)
Western blot analysis of Dynamin on PC12 cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-Dynamin.
Dynamin (clone 41) staining on rat brain. Formalin fixed paraffin section without citrate buffer pretreatment. 40X.
Western blot analysis of Dynamin on PC12 cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-Dynamin.
Dynamin (clone 41) staining on rat brain. Formalin fixed paraffin section without citrate buffer pretreatment. 40X.
Product Details
BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse, Dog, Chicken (Tested in Development)
Mouse IgG1
Rat Dynamin I aa. 698-851
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
100 kDa
250 µg/ml
AB_397640
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml .
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610245 Rev. 1
Antibody Details
41/Dynamin
Dynamin is a nerve terminal phosphoprotein with intrinsic GTPase activity which plays an important role in endocytosis. This GTPase activity is stimulated in vitro by microtubules, SH3 domain-containing proteins, phospholipids, and Protein Kinase C (PKC)-mediated phosphorylation. There are at least two distinct dynamin genes in mammals, which show 79% identity, encoding proteins Dynamin I and Dynamin II. Dynamin I is expressed almost exclusively in the central nervous system while Dynamin II expression is ubiquitous. Depolarization stimulates synaptic vesicle recycling and Dynamin I is subsequently dephosphorylated. This rapid dephosphorylation is mediated by calcineurin which acts as a switch for depolarization-initiated synaptic vesicle endocytosis. The C-terminus of dynamin contains two clusters of proline-rich SH3 domain binding proline motifs which interact with known SH3 domain proteins during tyrosine kinase receptor activation. It is reported that clone 41 detects both dynamin I and dynamin II.
610245 Rev. 1
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610245 Rev.1
Citations & References
View product citations for antibody "610245" on CiteAb
Development References (5)
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Hayes S, Chawla A, Corvera S. TGF beta receptor internalization into EEA1-enriched early endosomes: role in signaling to Smad2. J Cell Biol. 2002; 158(7):1239-1249. (Clone-specific: Immunofluorescence). View Reference
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Kalthoff C, Groos S, Kohl R, Mahrhold S, Ungewickell EJ. Clint: a novel clathrin-binding ENTH-domain protein at the Golgi. Mol Biol Cell. 2002; 13(11):4060-4073. (Clone-specific). View Reference
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Ochoa GC, Slepnev VI, Neff L, et al. A functional link between dynamin and the actin cytoskeleton at podosomes. J Cell Biol. 2000; 150(2):377-389. (Clone-specific: Western blot). View Reference
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Scaife R, Gout I, Waterfield MD, Margolis RL. Growth factor-induced binding of dynamin to signal transduction proteins involves sorting to distinct and separate proline-rich dynamin sequences. EMBO J. 1994; 13(11):2574-2582. (Biology). View Reference
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Schmidlin F, Dery O, DeFea KO, et al. Dynamin and Rab5a-dependent trafficking and signaling of the neurokinin 1 receptor. J Biol Chem. 2001; 276(27):25427-25437. (Clone-specific: Immunofluorescence, Western blot). View Reference
610245 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.