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Purified Mouse Anti-Human CD49e
Purified Mouse Anti-Human CD49e
Western blot analysis of CD49e (Integrin α5) on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the anti-human CD49e antibody.
Western blot analysis of CD49e (Integrin α5) on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the anti-human CD49e antibody.
Product Details
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BD Transduction Laboratories™
Integrin α5
Human (QC Testing)
Mouse IgG2a
Human Integrin α5 aa. 853-1002
Western blot (Routinely Tested), Immunoprecipitation (Tested During Development), Immunofluorescence, Immunohistochemistry (Not Recommended)
150 kDa
250 µg/ml
AB_397964
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
610633 Rev. 2
Antibody Details
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1/CD49e

Integrins are membrane receptors that mediate cell-cell or cell-matrix adhesion. They are transmembrane heterodimers composed of α and β subunits and are connected to the cytoskeleton. At least 20 integrins, formed from combinations of 12 α and 9 β subunits, have been reported. Many of these have been implicated as transducers of molecular signals. The integrin α5 subunit is 1049 amino acids with a molecular weight of 150-165 kDa. α5 and β1 form a functional fibronectin receptor which is important for vascular development in mice. Both subunits of the fibronectin receptor are heavily glycosylated in their extracellular domains. This modification is essential for proper cell attachment to basal membranes. Integrin α5 has been reported to suppress apoptosis by a Bcl-2 pathway and the C-terminal region is critical for cell motility and cytoskeletal rearrangements.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610633 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610633 Rev.2
Citations & References
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View product citations for antibody "610633" on CiteAb

Development References (5)

  1. Alahari SK, Lee JW, Julian RL. Nischarin, a novel protein that interacts with the integrin α5 subunit and inhibits cell migration. J Cell Biol. 2000; 151:1141-1154. (Biology: Western blot).
  2. Aplin AE, Short SM, Juliano RL. Anchorage-dependent regulation of the mitogen-activated protein kinase cascade by growth factors is supported by a variety of integrin alpha chains. J Biol Chem. 1999; 274(44):31223-31228. (Biology: Western blot). View Reference
  3. Bauer JS, Varner J, Schreiner C, Kornberg L, Nicholas R, Juliano RL. Functional role of the cytoplasmic domain of the integrin alpha 5 subunit. J Cell Biol. 1993; 122(1):209-221. (Biology). View Reference
  4. Sottile J, Hocking DC. Fibronectin polymerization regulates the composition and stability of extracellular matrix fibrils and cell-matrix adhesions. Mol Biol Cell. 2002; 13(10):3546-3559. (Biology: Immunofluorescence). View Reference
  5. Zheng M, Fang H, Hakomori S. Functional role of N-glycosylation in alpha 5 beta 1 integrin receptor. De-N-glycosylation induces dissociation or altered association of alpha 5 and beta 1 subunits and concomitant loss of fibronectin binding activity. J Biol Chem. 1994; 269(16):12325-12331. (Biology). View Reference
View All (5) View Less
610633 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.