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Western blot analysis of CD49e (Integrin α5) on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the anti-human CD49e antibody.
BD Transduction Laboratories™ Purified Mouse Anti-Human CD49e
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Integrins are membrane receptors that mediate cell-cell or cell-matrix adhesion. They are transmembrane heterodimers composed of α and β subunits and are connected to the cytoskeleton. At least 20 integrins, formed from combinations of 12 α and 9 β subunits, have been reported. Many of these have been implicated as transducers of molecular signals. The integrin α5 subunit is 1049 amino acids with a molecular weight of 150-165 kDa. α5 and β1 form a functional fibronectin receptor which is important for vascular development in mice. Both subunits of the fibronectin receptor are heavily glycosylated in their extracellular domains. This modification is essential for proper cell attachment to basal membranes. Integrin α5 has been reported to suppress apoptosis by a Bcl-2 pathway and the C-terminal region is critical for cell motility and cytoskeletal rearrangements.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (5)
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Alahari SK, Lee JW, Julian RL. Nischarin, a novel protein that interacts with the integrin α5 subunit and inhibits cell migration. J Cell Biol. 2000; 151:1141-1154. (Biology: Western blot).
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Aplin AE, Short SM, Juliano RL. Anchorage-dependent regulation of the mitogen-activated protein kinase cascade by growth factors is supported by a variety of integrin alpha chains. J Biol Chem. 1999; 274(44):31223-31228. (Biology: Western blot). View Reference
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Bauer JS, Varner J, Schreiner C, Kornberg L, Nicholas R, Juliano RL. Functional role of the cytoplasmic domain of the integrin alpha 5 subunit. J Cell Biol. 1993; 122(1):209-221. (Biology). View Reference
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Sottile J, Hocking DC. Fibronectin polymerization regulates the composition and stability of extracellular matrix fibrils and cell-matrix adhesions. Mol Biol Cell. 2002; 13(10):3546-3559. (Biology: Immunofluorescence). View Reference
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Zheng M, Fang H, Hakomori S. Functional role of N-glycosylation in alpha 5 beta 1 integrin receptor. De-N-glycosylation induces dissociation or altered association of alpha 5 and beta 1 subunits and concomitant loss of fibronectin binding activity. J Biol Chem. 1994; 269(16):12325-12331. (Biology). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.