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Purified Mouse Anti- Calcineurin
Purified Mouse Anti- Calcineurin
Western blot analysis of calcineurin. Lysate from A-431 cells was probed with anti-calcineurin (clone G182-1847) at concentrations of 1.0 (lane 1), 0.2 (lane 2), and 0.04 µg/ml (lane 3). The 61 kDa A subunit of calcineurin is identified.
Western blot analysis of calcineurin. Lysate from A-431 cells was probed with anti-calcineurin (clone G182-1847) at concentrations of 1.0 (lane 1), 0.2 (lane 2), and 0.04 µg/ml (lane 3). The 61 kDa A subunit of calcineurin is identified.
Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG1
Human β1 isoform of calcineurin A Subunit aa. 349-505
Western blot (Routinely Tested), Immunoprecipitation (Tested During Development)
61 kDa
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

Applications include immunoprecipitation (1-2 µg/ml) and western blot analysis (1-2 µg/ml). Jurkat human T cells (ATCC TIB-152)2, HeLa human carcinoma cells (ATCC CCL-2), and A-431 human epidermal carcinoma cells (ATCC CRL-1555) are suggested as positive controls. A431 cell lysate (Cat. No. 611447) and HeLa cell lysate (Cat. No. 611449) are also available as ready-to-use positive western blot control.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
556350 Rev. 8
Antibody Details
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Calcineurin is a Ca2+- and calmodulin-dependent serine/threonine phosphatase which consists of two subunits. The A subunit (~61 kDa) is catalytic and has calmodulinbinding activity; the B subunit (~19 kDa), which is myristylated at its N-terminus, has Ca[2+]-binding activity.  Calcineurin is expressed ubiquitously in eukaryotic cells, including yeast. The protein is highly expressed in mammalian brain, and has been implicated in a number of calcium-dependent pathways in the nervous system, including regulation of neurotransmitter release. Calcineurin also plays a key role in T cell receptor-mediated signal transduction pathways. Calcineurin dephosphorylates components of NF-AT (Nuclear Factor of Activated T cells), allowing NF-AT to translocate to the nucleus where it participates with AP-1 in activation of multiple cytokine and surface receptor genes. Inhibition of calcineurin by immunosuppressive agents such as cyclosporin A and FK506 correlates with the suppression of early T cell activation events. These immunosuppressive agents bind to specific intracellular proteins, called immunophilins, to form complexes that inhibit calcineurin's phosphatase activity. In the presence of these complexes, NF-AT remains phosphorylated and thus cannot translocate to the nucleus. A novel protein, Cain (calcineurin inhibitor), has been described which displays a similar pattern of neuronal and brain expression and has been shown to bind and inhibit calcineurin activity in vivo.

G182-1847 recognizes the α and β isoforms of the A subunit of calcineurin (~61 kDa). It reacts with an epitope in the autoinhibitory domain. It does not recognize the B subunit of calcineurin. A recombinant tag-fusion protein containing amino acids 349-505 of the human β1 isoform of calcineurin A subunit was used as immunogen.

556350 Rev. 8
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
556350 Rev.8
Citations & References
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View product citations for antibody "556350" on CiteAb

Development References (3)

  1. Fruman DA, Klee CB, Bierer BE, Burakoff SJ. Calcineurin phosphatase activity in T lymphocytes is inhibited by FK 506 and cyclosporin A. Proc Natl Acad Sci U S A. 1992; 89(9):3686-3690. (Biology). View Reference
  2. Husi H, Luyten MA, Zurini MG. Mapping of the immunophilin-immunosuppressant site of interaction on calcineurin. J Biol Chem. 1994; 269(19):14199-14204. (Biology). View Reference
  3. Lai MM, Burnett PE, Wolosker H, Blackshaw S, Snyder SH. Cain, a novel physiologic protein inhibitor of calcineurin. J Biol Chem. 1998; 273(29):18325-18331. (Biology). View Reference
556350 Rev. 8

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.