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BD Transduction Laboratories™ Purified Mouse Anti-PKCδ
Clone 14/PKC delta (RUO)
Western blot analysis of PKCδ on rat brain lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of PKCδ.
Rat brain zinc-fixed paraffin-embedded tissue
Purkinje Cells in rat cerebellum, formalin-fixed paraffin-embedded tissue, citrate pre-treatment, 40X
Western blot analysis of PKCδ on rat brain lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of PKCδ.
Rat brain zinc-fixed paraffin-embedded tissue
Purkinje Cells in rat cerebellum, formalin-fixed paraffin-embedded tissue, citrate pre-treatment, 40X
Product Details
BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse (Tested in Development)
Mouse IgG2b
Human PKCδ aa. 114-289
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
78 kDa
250 µg/ml
AB_397780
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610398 Rev. 2
Antibody Details
14/PKC delta
The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases. At least eleven isozymes have been described. These proteins are products of multiple genes and alternative splicing. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester. However, the novel PKC (nPKC) subfamily members (δ, ε, η, and θ isoforms) and the atypical PKC (aPKC) subfamily members (ζ, ι, and λ isoforms) are Ca2+ independent and lack the C2 domain. The aPKC members are unique in that their activity is independent of diacylglycerols and phorbol esters. They also lack one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC. The C-terminal region of PKC contains the catalytic domain. PKCδ is involved in myeloid differentiation, as well as in the secretory response of antigen-stimulated rat basophilic RBL 2H3 cells. Overexpression and subsequent stimulation of PKCδ leads to cell cycle arrest in CHO cells and complete growth inhibition of NIH 3T3 cells. PKCδ is the most abundant isoform in hemopoietic cells and is highly expressed in many other organs and tissues. This suggests that PKCδ may be one of the major PKC isozymes in mammalian cells.
610398 Rev. 2
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610398 Rev.2
Citations & References
View product citations for antibody "610398" on CiteAb
Development References (5)
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Bell RM, Burns DJ. Lipid activation of protein kinase C. J Biol Chem. 1991; 266(8):4661-4664. (Biology). View Reference
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Blass M, Kronfeld I, Kazimirsky G, Blumberg PM, Brodie C. Tyrosine phosphorylation of protein kinase Cdelta is essential for its apoptotic effect in response to etoposide. Mol Cell Biol. 2002; 22(1):182-195. (Clone-specific: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
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Larsen EC, Ueyama T, Brannock PM, et al. A role for PKC-epsilon in Fc gammaR-mediated phagocytosis by RAW 264.7 cells. J Cell Biol. 2002; 159(6):939-944. (Clone-specific: Immunofluorescence, Western blot). View Reference
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Nishizuka Y. The molecular heterogeneity of protein kinase C and its implications for cellular regulation. Nature. 1988; 334(6184):661-665. (Biology). View Reference
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Ringshausen I, Schneller F, Bogner C, et al. Constitutively activated phosphatidylinositol-3 kinase (PI-3K) is involved in the defect of apoptosis in B-CLL: association with protein kinase Cdelta. Blood. 2002; 100(10):3741-3748. (Clone-specific: Immunoprecipitation). View Reference
610398 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.