-
Your selected country is
United Kingdom
- Change country/language
Old Browser
This page has been recently translated and is available in French now.
Looks like you're visiting us from {countryName}.
Would you like to stay on the current country site or be switched to your country?
Western blot analysis of Ezrin on MDCK lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-Ezrin.
Immunofluorescent staining of HeLa cells with anti-Ezrin antibody.
BD Transduction Laboratories™ Purified Mouse Anti-Ezrin
BD Transduction Laboratories™ Purified Mouse Anti-Ezrin
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
First described as an 80kDa protein concentrated in the apical cytoskeletal region of intestinal brush border cells, ezrin is now recognized as a major substrate of protein tyrosine kinases, such as the epidermal growth factor (EGF) tyrosine kinase. Ezrin is expressed at high levels in intestine, kidney, and placenta. In placenta, ezrin is present as monomers and non-covalent oligomers in tight association with actin microfilaments. In the human epidermoid carcinoma cell line A431, microvilli-like structures appear within 30 seconds after the addition of EGF. These structures give way to membrane ruffles after 2-5 minutes, followed by cell rounding after 10-20 minutes. At the same time, ezrin is recruited into these structures and oligomers are formed following its tyrosine phosphorylation. It is thought that tyrosine phosphorylation triggers the formation of ezrin oligomers.
Development References (5)
-
Anastasiadis PZ, Moon SY, Thoreson MA, et al. Inhibition of RhoA by p120 catenin. Nat Cell Biol. 2000; 2(9):637-644. (Clone-specific: Immunofluorescence). View Reference
-
Berryman M, Gary R, Bretscher A. Ezrin oligomers are major cytoskeletal components of placental microvilli: a proposal for their involvement in cortical morphogenesis. J Cell Biol. 1995; 131(5):1231-1242. (Biology). View Reference
-
Defacque H, Egeberg M, Habermann A, et al. Involvement of ezrin/moesin in de novo actin assembly on phagosomal membranes. EMBO J. 2000; 19(2):199-212. (Clone-specific: Western blot). View Reference
-
Mohler PJ, Kreda SM, Boucher RC, Sudol M, Stutts MJ, Milgram SL. Yes-associated protein 65 localizes p62(c-Yes) to the apical compartment of airway epithelia by association with EBP50. J Cell Biol. 1999; 147(4):879-890. (Clone-specific: Immunofluorescence). View Reference
-
Perez OD, Kinoshita S, Hitoshi Y, et al. Activation of the PKB/AKT pathway by ICAM-2. Immunity. 2002; 16(1):51-65. (Clone-specific: Immunoprecipitation, Western blot). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.