Description
Interferon-γ (IFN-γ) is a potent multifunctional cytokine which is secreted by activated NK cells and CD4+TCRαβ+, CD8+TCRαβ+, and TCRγδ+ T cells and exerts its biological effects through specific binding to a single class of high affinity receptors. In addition to its antiviral effects, IFN-γ upregulates a number of lymphoid cell functions including the antimicrobial and antitumor responses of macrophages, NK cells, and neutrophils. In addition, IFN-γ can exert strong regulatory influences on the proliferation, differentiation, and effector responses of B cell and T cell subsets. These influences involve the action of IFN-γ to boost MHC class I and II expression by antigen-presenting cells as well as direct effects on B cells and T cells themselves. Recombinant rat IFN-γ (Cat. No. 550072) is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution and bovine serum albumin, with no preservatives. Recombinant rat IFN-γ is ≥ 95% pure as determined by SDS-PAGE and an absorbance assay based on the Beers-Lambert law. The endotoxin level is ≤ 0.1 ng/µg of rat IFN-γ, as measured in a chromegenic LAL assay.
Preparation And Storage
Recommended Assay Procedures
Upon initial thawing, recombinant rat IFN-γ (Cat. No. 550072) should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/mL carrier protein, such as human or bovine albumin, aliquoted and stored at -80°C. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. This product should not be diluted to less than 100 μg/mL for long-term storage. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may have an undesired influence on experimental results due to toxicity, high endotoxin levels or possible blocking activity.
Bioassay: Investigators are advised that the Bioassay application is not routinely test for this material and are highly encouraged to both titrate this material and include appropriate controls in relevant experiments. An activity range of 0.1 - 1.0 x 10^8 units/mg, encompassing an ED50= 0.1 - 1.0 ng/mL, has previously been reported using L929 indicator cells for proliferation, with a unit defined as the amount of material needed to stimulate a half-maximal response at cytokine saturation.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Development References (3)
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Farrar MA, Schreiber RD. The molecular cell biology of interferon-gamma and its receptor. Annu Rev Immunol. 1993; 11:571-611. (Biology). View Reference
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Gray PW, Goeddel DV. Cloning and expression of murine immune interferon cDNA. Proc Natl Acad Sci U S A. 1983; 80(19):5842-5846. (Biology). View Reference
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Green JA, Yeh TJ, Overall JC. Rapid, quantitative, semiautomated assay for virus-induced and immune human interferons. J Clin Microbiol. 1980; 12(3):433-438. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
