Description
Interleukin-2 (IL-2) is a cytokine which was first described and characterized based upon its potent ability to modulate lymphocyte reactivity and promote long term in vitro culture of antigen-specific effector T lymphocytes. It is responsible for the activities previously ascribed to T cell growth factor, thymocyte stimulating factor, helper-factor and T cell replacing factor, to name a few. IL-2 has also been reported to act on neuronal cells. Mouse IL-2 is a 17.2 kD protein containing 149 amino acid residues. Recombinant mouse IL-2 (Cat. No. 550069) is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution and bovine serum albumin, with no preservatives. Recombinant mouse IL-2 is ≥ 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law. The endotoxin level is ≤ 0.1 ng/µg of mouse IL-2, as measured in a chromegenic LAL assay.
Preparation And Storage
Recommended Assay Procedures
Upon initial thawing, recombinant mouse IL-2 (Cat. No. 550069) should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile netural buffer containing not less than 0.5 - 10 mg/mL carrier protein, such as human or bovine serum albumin, aliquoted and stored at -80°C. For use as an ELISA standard, carrier protein concentrations of 5 - 10 mg/mL are recommended. For in vtiro biological assays, carrier protein concentrations of 1 mg/mL are suggested. Failure to add carrier protein or store at the indicated temperatures may result in a loss of activity. This product should not be diluted to less than 10 µg/mL for long term storage. Carrier proteins should be pre-screened for possible effects in each investigator's experimental system. Carrier proteins may have an undesired influence on experimental results due to toxicity, high endotoxin levels or possible blocking activity.
ELISA Standard: Mouse IL-2 is useful as a quantitative standard for measuring mouse IL-2 protein levels in an IL-2 specific sandwich ELISA with the purified JES6-1A12 antibody (Cat. No. 554424) as a capture antibody and the biotinylated clone JES6-5H4 (Cat. No. 554426) as the detection antibody. To obtain linear standard curves, doubling dilutions of the mouse IL-2 standard from ~2,000 to 15 pg/mL should be included with each ELISA plate. This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems, not for assay of serum or plasma samples. For measuring IL-2 in serum or plasma, the BD OptEIA™ Mouse IL-2 Set (Cat. No. 555148) is specially formulated and recommended.
Bioassay: Investigators are advised that the Bioassay application is not routinely tested for this material and are highly encouraged to both titrate this material and include appropriate controls in relevant experiments. An activity range of 0.1 - 1.0 x 10^9 units/mg, encompassing an
ED50= 10 - 100 pg/mL, has previously been reported using CTLL-2 indicator cells for proliferation, with a unit defined as the amount of material needed to stimulate a half-maximal response at cytokine saturation.
Blocking: Recombinant mouse IL-2 (Cat. No. 550069) can be useful as a blocking control for flow cytometric analysis when used with fluorochrome conjugated antibodies, such as PE-conjugated JES-5H4 (Cat. No. 554428). Investigators are advised that the blocking application is not routinely tested for this material. Intracellular cytokine staining techniques and use of blocking controls are described in detail by C.Prussin and D.Metcalfe.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Development References (8)
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Awatsuji H, Furukawa Y, Nakajima M, Furukawa S, Hayashi K.. Interleukin-2 as a neurotrophic factor for supporting the survival of neurons cultured from various regions of fetal rat brain. J Neurosci Res. 1993; 35(3):305-311. (Biology). View Reference
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Gillis S, Ferm MM, Ou W, Smith KA. T cell growth factor: parameters of production and a quantitative microassay for activity. J Immunol. 1978; 120(6):2027-2032. (Biology). View Reference
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Kashima N, Nishi-Takaoka C, Fujita T, et al. Unique structure of murine interleukin-2 as deduced from cloned cDNAs. Nature. 1985; 313(6001):402-404. (Biology). View Reference
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Mochizuki DY, Watson J, Gillis S. Biochemical separation of interleukin 2. J Immunol Methods. 1980; 39(3):185-201. (Biology). View Reference
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Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology: IC/FCM Block). View Reference
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Sarder M, Saito H, Abe K. Interleukin-2 promotes survival and neurite extension of cultured neurons from fetal rat brain. Brain Res. 1993; 625(2):347-350. (Biology). View Reference
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Watson J, Mochizuki D. Interleukin 2: a class of T cell growth factors. Immunol Rev. 1980; 51:257-278. (Biology). View Reference
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Yokota T, Arai N, Lee F, Rennick D, Mosmann T, Arai K. Use of a cDNA expression vector for isolation of mouse interleukin 2 cDNA clones: expression of T-cell growth-factor activity after transfection of monkey cells. Proc Natl Acad Sci U S A. 1985; 82(1):68-72. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
