Interleukin 12 (IL-12) is a potent regulator of cell-mediated immune responses. Biologically active IL-12 is secreted by activated B lymphocytes and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD (p35) and 40 kD (p40) subunits. The IL-12 p40 monomer shares amino acid sequence homology with the IL-6 receptor. It has been reported that activated PBMC produce a large excess of IL-12 p40 monomer over the bioactive p70 heterodimer. The IL-12 p40 monomer has been reported to inhibit binding of IL-12 p70 to the IL-12 receptor, but with 20X less effectiveness than the IL-12 p40 homodimer.
Formulation and Purity:
Recombinant mouse IL-12 p40 is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered Dulbecco's PBS (pH 7.2) and no preservatives. The recombinant mouse IL-12, p40 is > 95% pure, as determined by SDS-PAGE, and an absorption assay based on the Beer-Lambert law. The recombinant is not tested for endotoxin or sterility.