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HRP Rat Anti-Mouse IgM
Product Details
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BD Pharmingen™
Mouse (QC Testing)
Rat IgG2a, κ
Not reported
ELISA Detection (Routinely Tested)
Aqueous buffered solution containing BSA and ProClin™ 150 as preservative.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with HRP under optimum conditions, and unconjugated antibody and free HRP were removed.

Recommended Assay Procedures

ELISA: For detection of mouse IgM by sandwich ELISA, this antibody may be used as the detection antibody at a ~1:1000 dilution coupled with purified rat anti-mouse IgM antibody (clone R6-60.2) (Cat.No. 553405) as the capture antibody.  Purified mouse IgM (Cat.No. 553472) may be used as the ELISA standard.

Warning:  HRP Rat Anti-Mouse IgM contains 0.004% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).

Hazard statements

May cause an allergic skin reaction.

Precautionary statements

Wear protective gloves / eye protection.

Wear protective clothing.

Avoid breathing mist/vapours/spray.

If skin irritation or rash occurs: Get medical advice/attention.

IF ON SKIN: Wash with plenty of water.

Dispose of contents/container in accordance with local/regional/national/international regulations.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. ProClin is a trademark of Rohm and Haas Company.
550588 Rev. 8
Antibody Details
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The II/41 clone has been reported to react specifically with mouse IgM of Igh-C[a] and Igh-C[b] haplotypes. It has been reported not to react with other Ig isotypes. In addition, the II/41 clone has been reported not to stimulate B-cell proliferation.

550588 Rev. 8
Format Details
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Horseradish Peroxidase is an enzyme that is capable of cleaving a substrate into hydrogen peroxide which can then be detected in solution or in situ. Antibodies, avidins, and other specific binding molecules conjugated with horseradish peroxidase, in conjunction with an appropriate substrate system, can be used for multiple ELISA or Immunohistochemical applications.
550588 Rev.8
Citations & References
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View product citations for antibody "550588" on CiteAb

Development References (1)

  1. Laszlo G, Hathcock KS, Dickler HB, Hodes RJ. Characterization of a novel cell-surface molecule expressed on subpopulations of activated T and B cells. J Immunol. 1993; 150(12):5252-5262. (Biology). View Reference
550588 Rev. 8

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.