Preparation And Storage
Recommended Assay Procedures
ELISA Detection: The biotinylated BVD6-24G2 antibody (Cat. No. 554390) is useful as a detection antibody for a sandwich ELISA for measuring mouse IL-4 protein levels. The biotinylated BVD6-24G2 antibody can be paired with the purified BVD4-1D11 IL-4 antibody (Cat. No. 554387) or the purified 11B11 IL-4 antibody (Cat. No. 554434) as the capture antibody, with recombinant mouse IL-4 (Cat. No. 550067) as the standard. The biotinylated BVD6-24G2 antibody should be titrated 0.5 - 2.0 µg/ml to determine optimal concentration for ELISA detecton. To obtain linear standard curves, doubling dilutions of mouse IL-4 protein ranging from ~2000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit the protocols section or chapter on ELISA in the Immune Function Handbook, both of which are posted on our website, www.bdbiosciences.com.
Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum or plasma samples. For measuring mouse IL-4 in serum or plasma our mouse IL-4 OptEIA set (Cat. No. 555232) is especially formulated and recommended.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
The BVD6-24G2 antibody reacts with mouse interleukin-4 (IL-4). The immunogen used to generate the BVD6-24G2 hybridoma was recombinant IL-4. This is a non-neutralizing antibody.
This antibody is routinely tested by ELISA Detection. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (5)
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Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Clone-specific: ELISA). View Reference
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Bogen SA, Fogelman I, Abbas AK. Analysis of IL-2, IL-4, and IFN-gamma-producing cells in situ during immune responses to protein antigens. J Immunol. 1993; 150(10):4197-4205. (Clone-specific: ELISA). View Reference
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Chatelain R, Varkila K, Coffman RL. IL-4 induces a Th2 response in Leishmania major-infected mice. J Immunol. 1992; 148(4):1182-1187. (Clone-specific: ELISA). View Reference
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Sander B, Hoiden I, Andersson U, Moller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993; 166(2):201-214. (Clone-specific: ELISA). View Reference
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Shirai A, Sierra V, Kelly CI, Klinman DM. Individual cells simultaneously produce both IL-4 and IL-6 in vivo. Cytokine. 1994; 6(3):329-336. (Clone-specific: ELISA). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
