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BD Horizon™ V450 Mouse Anti-Human CD11c
Clone B-ly6 (RUO)
Analysis of CD11c in human peripheral blood lymphocytes. Whole blood was stained with
BD Horizon™ V450 Mouse Anti-Human CD11c and compared to whole blood stained with BD Horizon™ V450 Mouse IgG1, κ Isotype Control (clone MOPC-21, Cat. No. 560373). The isotype control is represented by a dashed line and the V450 Mouse Anti-Human CD11c by the solid line. Lymphocytes were selected by light scatter profile. Flow cytometry was performed on a BD LSR™ II flow cytometry system.
Analysis of CD11c in human peripheral blood monocytes. Whole blood was stained with BD Horizon™ V450 Mouse Anti-Human CD11c and compared to whole blood stained with BD Horizon™ V450 Mouse IgG1, κ Isotype Control (clone MOPC-21, Cat. No. 560373). The isotype control is represented by a dashed line and the V450 Mouse Anti-Human CD11c by the solid line. Monocytes were selected by light scatter profile. Flow cytometry was performed on a BD LSR™ II flow cytometry system.
Analysis of CD11c in human peripheral blood lymphocytes. Whole blood was stained with
BD Horizon™ V450 Mouse Anti-Human CD11c and compared to whole blood stained with BD Horizon™ V450 Mouse IgG1, κ Isotype Control (clone MOPC-21, Cat. No. 560373). The isotype control is represented by a dashed line and the V450 Mouse Anti-Human CD11c by the solid line. Lymphocytes were selected by light scatter profile. Flow cytometry was performed on a BD LSR™ II flow cytometry system.
Analysis of CD11c in human peripheral blood monocytes. Whole blood was stained with BD Horizon™ V450 Mouse Anti-Human CD11c and compared to whole blood stained with BD Horizon™ V450 Mouse IgG1, κ Isotype Control (clone MOPC-21, Cat. No. 560373). The isotype control is represented by a dashed line and the V450 Mouse Anti-Human CD11c by the solid line. Monocytes were selected by light scatter profile. Flow cytometry was performed on a BD LSR™ II flow cytometry system.
Product Details
BD Horizon™
ITGAX; AlphaX integrin chain; Axb2; Integrin alpha-X; CR4; Leu M5; SLEB6
Human (QC Testing)
Mouse BALB/c IgG1, κ
Spleen Cells from Human with Hairy Cell Leukemia
Flow cytometry (Routinely Tested)
5 µl
IV N012
AB_1645557
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ V450 under optimum conditions, and unreacted BD Horizon™ V450 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- BD Horizon V450 has a maximum absorption of 406 nm and maximum emission of 450 nm. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
560369 Rev. 1
Antibody Details
B-ly6
The B-ly6 monoclonal antibody specifically binds to the 150 kDa adhesion glycoprotein CD11c (p150, integrin α chain). CD11c is expressed on dendritic cells, monocytes, macrophages, granulocytes, NK cells and subsets of B and T cells. It associates with CD18 to form the CD11c/CD18 complex that binds fibrinogen and has been reported to be a receptor for iC3b and ICAM-1. Reports indicate that CD11c/CD18 plays a role as an adhesion molecule that mediates cellular binding to ligands expressed on stimulated epithelium and endothelium.
The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406 nm and has an Em Max at 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.
560369 Rev. 1
Format Details
V450
BD Horizon™ V450 Dye is part of the BD Horizon™ violet family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 405-nm and an emission maximum (Em Max) at 450-nm. BD Horizon™ V450, driven by BD innovation, is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450-nm (e.g., a 450/50-nm bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
V450
405 nm
450 nm
560369 Rev.1
Citations & References
View product citations for antibody "560369" on CiteAb
Development References (3)
-
Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
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Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
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Stacker SA, Springer TA. Leukocyte integrin P150,95 (CD11c/CD18) functions as an adhesion molecule binding to a counter-receptor on stimulated endothelium. J Immunol. 1991; 146(2):648-655. (Biology). View Reference
560369 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.