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Purified NA/LE Rat Anti-Mouse IL-10
Product Details
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BD Pharmingen™
Interleukin-10; Il10; CSIF; Cytokine synthesis inhibitory factor
Mouse (QC Testing)
Rat IgG2b
Recombinant mouse IL-10
Intracellular staining (flow cytometry) (Routinely Tested), ELISA, Neutralization (Tested During Development)
1.0 mg/ml
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.

Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Recommended Assay Procedures

ELISA:  Biotin Rat Anti-Mouse IL-10 (Clone JES5-16E3, Cat. No. 554465) is useful as a detection antibody for sandwich ELISA for measuring mouse IL-10 protein levels and can be paired with Purified Rat Anti-Mouse IL-10 (Clone JES5-2A5, Cat. No 551215) as the capture antibody along with recombinant mouse IL-10 (Cat. No. 550070) as the standard.  For measuring mouse IL-10 in complex biological fluids, such as serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Mouse IL-10 ELISA Set (Cat. No. 555252).

Flow Cytometry: The JES5-16E3 antibody is useful for flow cytometric analysis to identify and enumerate IL-10 producing cells within mixed cell populations. Direct conjugates of the JES5-16E3 antibody are especially suitable for these experiments, such as PE Rat Anti-Mouse IL-10 (Cat. No. 554467).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
554463 Rev. 2
Antibody Details
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The JES5-16E3 monoclonal antibody specifically binds to the mouse cytokine, Interleukin-10 (IL-10). IL-10 is also known as Cytokine Synthesis Inhibitory Factor (CSIF). It is produced by various activated cell types including CD4+ T cells, CD8+ T cells, T regulatory cells, NK T cells, B1 B cells, NK cells, macrophages, dendritic cells, mast cells, granulocytes and keratinocytes. IL-10 plays a pivotal role in regulating immune responses and protecting the host from damage caused by inflammatory and autoimmune responses. IL-10 has numerous biological activities including the inhibition of cytokine synthesis by activated T cells, NK cells, monocytes, and macrophages. In the presence of accessory cells, IL-10 inhibits mitogen- or anti-CD3 induced proliferation of T lymphocytes.  IL-10 has also been shown to costimulate the development of thymocytes, B cell differentiation and the generation of cytotoxic T cells. The immunogen used to generate the JES5-16E3 hybridoma was recombinant mouse IL-10. JES5-16E3 is a neutralizing antibody.

554463 Rev. 2
Format Details
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NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
554463 Rev.2
Citations & References
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Development References (4)

  1. Andersson U, Andersson J. Immunolabeling of cytokine-producing cells in tissues and in suspension. In: Fradelizie D, Emelie D, ed. Cytokine Producing Cells. Paris: Inserm; 1994:32-49.
  2. Litton MJ, Sander B, Murphy E, O'Garra A, Abrams JS. Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B. J Immunol Methods. 1994; 175(1):47-58. (Immunogen). View Reference
  3. Sander B, Hoiden I, Andersson U, Moller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993; 166(2):201-214. (Immunogen). View Reference
  4. Schlaak JF, Schmitt E, Hüls C, Meyer zum Büschenfelde KH, Fleischer B. A sensitive and specific bioassay for the detection of human interleukin-10. J Immunol Methods. 1994; 168(1):49-54. (Clone-specific). View Reference
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554463 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.