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BD Pharmingen™ Purified Hamster Anti-Mouse CD3e
Clone 500A2 (RUO)
Immunohistochemical staining of T lymphocytes.
A frozen section from mouse spleen was stained with the Hamster Anti-Mouse CD3e(Cat. No. 550277) antibody. T lymphocytes can be identified by the intense brown labeling of their cell surface membranes (20X magnification).
Immunohistochemical staining of T lymphocytes.
A frozen section from mouse spleen was stained with the Hamster Anti-Mouse CD3e(Cat. No. 550277) antibody. T lymphocytes can be identified by the intense brown labeling of their cell surface membranes (20X magnification).
Product Details
BD Pharmingen™
CD3; CD3 epsilon; Cd3e; CD3ε; T3e
Mouse (QC Testing)
Syrian Hamster IgG2, κ
Mouse T-cell receptor
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-paraffin (Not Recommended)
62.5 µg/ml
AB_393573
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures
Immunohistochemistry: For optimal indirect immunohistochemical staining, this antibody should be titrated (e.g suggested starting dilutions of 1:10 to 1:50) and visualized via a three step staining procedure in combination with a Biotin Mouse Anti-Hamster IgG2 antibody (Cat. No. 554029) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880).
Investigators should note that this antibody is not recommended for paraffin-embedded sections.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- An isotype control should be used at the same concentration as the antibody of interest.
- This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Purified Hamster IgG2, λ1 Isotype Control RUO
Size
1 ML
Cat No.
550345
Streptavidin HRP RUO
Size
50 ML
Cat No.
550946
Biotin Mouse Anti-Syrian Hamster IgG2 RUO
Size
0.5 mg
Cat No.
554029
DAB Substrate Kit RUO
Size
500 Tests
Cat No.
550880
550277 Rev. 3
Antibody Details
500A2
The 500A2 monoclonal antibody specifically binds to the 25-kDa ε chain of the T-cell receptor-associated CD3 complex expressed on mouse thymocytes, mature T lymphocytes, and NKT cells. Plate-bound and soluble forms of the 500A2 antibody can activate T cells in vitro. Activation of a mouse T-cell clone by the 500A2 antibody can be blocked by Fab fragments of the GK1.5 anti-CD4 antibody. This suggests that the 500A2 antibody may bind an epitope on CD3e close to a site at which CD4 associates with the T-cell receptor. The 500A2 antibody reportedly does not to crossreact with rat leukocytes.
550277 Rev. 3
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
550277 Rev.3
Citations & References
View product citations for antibody "550277" on CiteAb
Development References (3)
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Allison JP, Havran WL, Poenie M, et al. Expression and function of CD3 on murine thymocytes. In: Kappler J, Davis M, ed. The T-Cell Receptor, UCLA Symposia, 73rd Edition. Los Angeles: 1988:33-45.
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Havran WL, Poenie M, Kimura J, Tsien R, Weiss A, Allison JP. Expression and function of the CD3-antigen receptor on murine CD4+8+ thymocytes. Nature. 1987; 330(6144):170-173. (Biology: (Co)-stimulation, Immunohistochemistry). View Reference
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Portoles P, Rojo J, Golby A, et al . Monoclonal antibodies to murine CD3 epsilon define distinct epitopes, one of which may interact with CD4 during T cell activation. J Immunol. 1989; 142(12):4169-4175. (Biology). View Reference
550277 Rev. 3
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.