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BD Pharmingen™ PE Rat Anti-Mouse TSLP Receptor
Clone 22H9 (RUO)
Flow cytometric analysis of TSLPR expression on mouse splenic leucocytes. Splenic leucocytes from a C56BL/6 mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with either PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; dashed line histogram) or PE Rat Anti-Mouse TSLPR antibody (Cat. No. 566673; solid line histogram) at 0.5 µg/test. The fluorescence histogram showing TSLPR expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Two-color flow cytometric analysis of TSLPR expression on mouse bone marrow cells. Bone marrow leucocytes from BALB/c mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™). The cells were then stained with BD Horizon™ BV421 Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 562922) and either PE Rat IgG2a, κ Isotype Control (Left Plot) or PE Rat Anti-Mouse TSLPR antibody (Right Plot) at 0.5 µg/test. Two-color flow cytometric contour plots showing the correlated expression of TSLPR (or Ig Isotype control staining) versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of viable bone marrow leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Flow cytometric analysis of TSLPR expression on mouse splenic leucocytes. Splenic leucocytes from a C56BL/6 mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with either PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; dashed line histogram) or PE Rat Anti-Mouse TSLPR antibody (Cat. No. 566673; solid line histogram) at 0.5 µg/test. The fluorescence histogram showing TSLPR expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Two-color flow cytometric analysis of TSLPR expression on mouse bone marrow cells. Bone marrow leucocytes from BALB/c mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™). The cells were then stained with BD Horizon™ BV421 Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 562922) and either PE Rat IgG2a, κ Isotype Control (Left Plot) or PE Rat Anti-Mouse TSLPR antibody (Right Plot) at 0.5 µg/test. Two-color flow cytometric contour plots showing the correlated expression of TSLPR (or Ig Isotype control staining) versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of viable bone marrow leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Product Details
BD Pharmingen™
TSLP receptor; Tslpr; Ly114; Crlf2; CRLM2; Tpte2
Mouse (QC Testing)
Rat IgG2a, κ
TSLP Receptor Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2739764
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO
Size
0.1 mg
Cat No.
553141
Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO
Size
0.5 mg
Cat No.
553142
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
Lysing Buffer RUO
Size
100 ML
Cat No.
555899
PE Rat IgG2a, κ Isotype Control RUO
Size
0.1 mg
Cat No.
553930
566673 Rev. 1
Antibody Details
22H9
The 22H9 monoclonal antibody specifically recognizes the Thymic stromal lymphopoietin protein receptor (TSLP Receptor or TSLPR) which is also known as Cytokine receptor-like molecule 2 (CRLM2), Lymphocyte antigen 114 (Ly114), Type I cytokine receptor delta 1, or Transmembrane phosphoinositide 3-phosphatase and tensin homolog 2 (Tpte2). This type I transmembrane glycoprotein is encoded by Crlf2 (Cytokine receptor-like factor 2) which belongs to the hematopoietin receptor gene superfamily. The functional, high affinity receptor complex for Thymic stromal lymphopoietin (TSLP) is comprised of the TSLP Receptor and Interleukin-7 receptor alpha chain (IL-7Rα/CD127). TSLP is produced by a variety of cell types including epithelial cells, keratinocytes, and stromal cells. The TSLP Receptor is variably expressed on epithelial cells and on various cell types of hematopoietic origin including thymocytes, T cells, NK cells, B cells, monocytes, macrophages, dendritic cells (DC), granulocytes, and mast cells. TSLP signaling through the heterodimeric TSLP Receptor complex induces Stat5 tyrosine phosphorylation and supports the maturation and differentiation of multiple different cells types including B and T cells as well as dendritic cells that promote type-2 (Th2-like) T cell responses.
566673 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
496 nm, 566 nm
576 nm
566673 Rev.1
Citations & References
View product citations for antibody "566673" on CiteAb
Development References (6)
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Isaksen DE, Baumann H, Zhou B, et al. Uncoupling of proliferation and Stat5 activation in thymic stromal lymphopoietin-mediated signal transduction.. J Immunol. 2002; 168(7):3288-94. (Clone-specific: Flow cytometry). View Reference
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Iseki M, Omori-Miyake M, Xu W, et al. Thymic stromal lymphopoietin (TSLP)-induced polyclonal B-cell activation and autoimmunity are mediated by CD4+ T cells and IL-4.. Int Immunol. 2012; 24(3):183-95. (Clone-specific: Flow cytometry). View Reference
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Kashyap M, Rochman Y, Spolski R, Samsel L, Leonard WJ. Thymic stromal lymphopoietin is produced by dendritic cells.. J Immunol. 2011; 187(3):1207-11. (Biology). View Reference
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Park LS, Martin U, Garka K, et al. Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor. J Exp Med. 2000; 192(5):659-670. (Biology). View Reference
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Rochman Y, Leonard WJ. The role of thymic stromal lymphopoietin in CD8+ T cell homeostasis.. J Immunol. 2008; 181(11):7699-705. (Clone-specific: Flow cytometry). View Reference
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Takai T. TSLP expression: cellular sources, triggers, and regulatory mechanisms.. Allergol Int. 2012; 61(1):3-17. (Biology). View Reference
566673 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.