BD Pharmingen™ PE Mouse anti-Mouse Nanog
Clone M55-312 (RUO)
Flow cytometry analysis of PE anti Nanog on mouse embryonic stem (ES) cells. Mouse ES-E14TG2a (ATCC, Cat. No. CRL-1821) cells were fixed (BD Cytofix™ buffer, Cat. No. 554655) for 10 minutes at 37 C, permeabilized with BD™ Phosflow Perm Buffer III (Cat. No. 558050) on ice for 30 minutes, and then stained with either PE Mouse anti-Nanog (solid line) or PE Mouse IgG1,k Isotype Control (Cat. No. 554680, dashed line). This antibody also works in BD™ Phosflow Perm Buffer I and II. Flow cytometry was performed on a BD FACSCanto™ II flow cytometry system.
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Flow cytometry analysis of PE anti Nanog on mouse embryonic stem (ES) cells. Mouse ES-E14TG2a (ATCC, Cat. No. CRL-1821) cells were fixed (BD Cytofix™ buffer, Cat. No. 554655) for 10 minutes at 37 C, permeabilized with BD™ Phosflow Perm Buffer III (Cat. No. 558050) on ice for 30 minutes, and then stained with either PE Mouse anti-Nanog (solid line) or PE Mouse IgG1,k Isotype Control (Cat. No. 554680, dashed line). This antibody also works in BD™ Phosflow Perm Buffer I and II. Flow cytometry was performed on a BD FACSCanto™ II flow cytometry system.
Product Details
BD Pharmingen™
Mouse (QC Testing)
Mouse BALB/c IgG1, κ
Mouse Nanog Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
20 µl
AB_1645245
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
Fixation Buffer RUO
Size
100 mL
Cat No.
554655
Perm Buffer III RUO
Size
125 mL
Cat No.
558050
PE Mouse IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
554680
560277 Rev. 1
Antibody Details
M55-312
The M55-312 monoclonal antibody reacts with mouse Nanog (named for Tir Na Nog, the land of the ever-young of Celtic mythology), which is a homeobox transcription factor required for the maintenance of the undifferentiated state of pluripotent stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF (Leukemia Inhibitory Factor) and BMP (Bone Morphogenic Protein). When Nanog expression is down-regulated, cell differentiation can proceed. Proteins that regulate Nanog expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.
560277 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
560277 Rev.1
Citations & References
View product citations for antibody "560277" on CiteAb
Development References (6)
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Chambers I, Colby D, Robertson M, et al. Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell. 2003; 113:643-655. (Biology). View Reference
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Chambers I. The molecular basis of pluripotency in mouse embryonic stem cells. Cloning Stem Cells. 2004; 6(4):386-391. (Biology). View Reference
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Mitsui K, Tokuzawa Y, Itoh H, et al. The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell. 2003; 113:631-642. (Biology). View Reference
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Pan G, Thomson JA. Nanog and transcriptional networks in embryonic stem cell pluripotency. Cell Res. 2007; 17:42-49. (Biology). View Reference
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Sun Y, Li H, Yang H, Rao MS, Zhan M. Mechanisms controlling embryonic stem cell self-renewal and differentiation. Crit Rev Eukaryot Gene Expr.. 2006; 16(3):211-231. (Biology). View Reference
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Suzuki A, Raya A, Kawakami Y, et al. Nanog binds to Smad1 and blocks bone morphogenetic protein-induced differentiation of embryonic stem cells. Proc Natl Acad Sci U S A. 2006; 103(27):10294-10299. (Biology). View Reference
560277 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.