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      FITC Mouse Anti-Human Light Chain λ
      FITC Mouse Anti-Human Light Chain λ
      Two color flow cytometric analysis of λ light chain expression on human peripheral blood lymphocytes. Whole blood was stained with FITC Mouse Anti-Human Light Chain λ (Cat. No. 555796/562053) and PE Mouse Anti-Human CD19 (Cat. No. 561741/555413). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The two-color dot plot depicting λ light chain versus CD19 expression was derived from gated events with the forward and side light-scattering characteristics of intact lymphocytes. Flow cytometry was performed on a BD FACScan™ system.
      FITC Mouse Anti-Human Light Chain λ
      Two color flow cytometric analysis of λ light chain expression on human peripheral blood lymphocytes. Whole blood was stained with FITC Mouse Anti-Human Light Chain λ (Cat. No. 555796/562053) and PE Mouse Anti-Human CD19 (Cat. No. 561741/555413). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The two-color dot plot depicting λ light chain versus CD19 expression was derived from gated events with the forward and side light-scattering characteristics of intact lymphocytes. Flow cytometry was performed on a BD FACScan™ system.
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      BD Pharmingen™
      Ig λ; IGL@; IGL; Immunoglobulin lambda locus
      Human (QC Testing)
      Mouse IgG1, κ
      Flow cytometry (Routinely Tested)
      20 µl
      AB_396130
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
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      Recommended Assay Procedures

      BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation).  When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.

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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      555796 Rev. 8
      Antibody Details
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      JDC-12

      The JDC-12 monoclonal antibody specifically binds to human immunoglobulin light chain, lambda (λ). It does not bind to immunoglobulin κ light chains or heavy chains.

      555796 Rev. 8
      Format Details
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      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      FITC
      Blue 488 nm
      494 nm
      518 nm
      555796 Rev.8
      Citations & References
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      View product citations for antibody "555796" on CiteAb

      Development References (2)

      1. Odendahl M, Jacobi A, Hansen A, et al. Disturbed peripheral B lymphocyte homeostasis in systemic lupus erythematosus. J Immunol. 2000; 165(10):5970-5979. (Clone-specific: Flow cytometry). View Reference
      2. Sembries S, Pahl H, Stilgenbauer S, Döhner H, Schriever F. Reduced expression of adhesion molecules and cell signaling receptors by chronic lymphocytic leukemia cells with 11q deletion.. Blood. 1999; 93(2):624-31. (Biology). View Reference
      555796 Rev. 8

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.