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BD Horizon™ BV421 Rat Anti-Mouse IL-2
Clone JES6-5H4 (RUO)
Two-color flow cytometric analysis of IL-2 expressed in activated CD4-positive splenocytes. MiCK-1 Mouse Cytokine Positive Control Cells (Cat No. 554652) were permeabilized using BD Perm/Wash™ Buffer (Cat. No. 554723). The permeabilized cells were stained with APC Rat Anti-Mouse CD4 (Cat. No. 553051/561091) and either BD Horizon™ BV421 Rat IgG2b Isotype Control (Cat No. 562603, Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse IL-2 (Cat No. 562969, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of IL-2 (or Ig Isotype control staining) versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Two-color flow cytometric analysis of IL-2 expressed in activated CD4-positive splenocytes. MiCK-1 Mouse Cytokine Positive Control Cells (Cat No. 554652) were permeabilized using BD Perm/Wash™ Buffer (Cat. No. 554723). The permeabilized cells were stained with APC Rat Anti-Mouse CD4 (Cat. No. 553051/561091) and either BD Horizon™ BV421 Rat IgG2b Isotype Control (Cat No. 562603, Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse IL-2 (Cat No. 562969, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of IL-2 (or Ig Isotype control staining) versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Two-color flow cytometric analysis of IL-2 expressed in activated CD4-positive splenocytes. MiCK-1 Mouse Cytokine Positive Control Cells (Cat No. 554652) were permeabilized using BD Perm/Wash™ Buffer (Cat. No. 554723). The permeabilized cells were stained with APC Rat Anti-Mouse CD4 (Cat. No. 553051/561091) and either BD Horizon™ BV421 Rat IgG2b Isotype Control (Cat No. 562603, Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse IL-2 (Cat No. 562969, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of IL-2 (or Ig Isotype control staining) versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Two-color flow cytometric analysis of IL-2 expressed in activated CD4-positive splenocytes. MiCK-1 Mouse Cytokine Positive Control Cells (Cat No. 554652) were permeabilized using BD Perm/Wash™ Buffer (Cat. No. 554723). The permeabilized cells were stained with APC Rat Anti-Mouse CD4 (Cat. No. 553051/561091) and either BD Horizon™ BV421 Rat IgG2b Isotype Control (Cat No. 562603, Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse IL-2 (Cat No. 562969, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of IL-2 (or Ig Isotype control staining) versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Two-color flow cytometric analysis of IL-2 expressed in activated CD4-positive splenocytes. MiCK-1 Mouse Cytokine Positive Control Cells (Cat No. 554652) were permeabilized using BD Perm/Wash™ Buffer (Cat. No. 554723). The permeabilized cells were stained with APC Rat Anti-Mouse CD4 (Cat. No. 553051/561091) and either BD Horizon™ BV421 Rat IgG2b Isotype Control (Cat No. 562603, Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse IL-2 (Cat No. 562969, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of IL-2 (or Ig Isotype control staining) versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Two-color flow cytometric analysis of IL-2 expressed in activated CD4-positive splenocytes. MiCK-1 Mouse Cytokine Positive Control Cells (Cat No. 554652) were permeabilized using BD Perm/Wash™ Buffer (Cat. No. 554723). The permeabilized cells were stained with APC Rat Anti-Mouse CD4 (Cat. No. 553051/561091) and either BD Horizon™ BV421 Rat IgG2b Isotype Control (Cat No. 562603, Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse IL-2 (Cat No. 562969, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of IL-2 (or Ig Isotype control staining) versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Two-color flow cytometric analysis of IL-2 expressed in activated CD4-positive splenocytes. MiCK-1 Mouse Cytokine Positive Control Cells (Cat No. 554652) were permeabilized using BD Perm/Wash™ Buffer (Cat. No. 554723). The permeabilized cells were stained with APC Rat Anti-Mouse CD4 (Cat. No. 553051/561091) and either BD Horizon™ BV421 Rat IgG2b Isotype Control (Cat No. 562603, Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse IL-2 (Cat No. 562969, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of IL-2 (or Ig Isotype control staining) versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Two-color flow cytometric analysis of IL-2 expressed in activated CD4-positive splenocytes. MiCK-1 Mouse Cytokine Positive Control Cells (Cat No. 554652) were permeabilized using BD Perm/Wash™ Buffer (Cat. No. 554723). The permeabilized cells were stained with APC Rat Anti-Mouse CD4 (Cat. No. 553051/561091) and either BD Horizon™ BV421 Rat IgG2b Isotype Control (Cat No. 562603, Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse IL-2 (Cat No. 562969, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of IL-2 (or Ig Isotype control staining) versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Product Details
BD Horizon™
Il2; Interleukin-2; T-cell growth factor; TCGF
Mouse (QC Testing)
Rat IgG2b
Mouse IL-2 Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2737923
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
- Brilliant Violet™ 421 is a trademark of Sirigen.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Perm/Wash Buffer RUO
Size
100 ML
Cat No.
554723
BV421 Rat IgG2b, κ Isotype Control RUO
Size
50 µg
Cat No.
562603
MiCK-1 Mouse Cytokine Positive Control Cells RUO
Size
1 ML
Cat No.
554652
APC Rat Anti-Mouse CD4 RUO
Size
25 mg
Cat No.
561091
APC Rat Anti-Mouse CD4 RUO
Size
0.1 mg
Cat No.
553051
566296 Rev. 1
Antibody Details
JES6-5H4
The JES6-5H4 monoclonal antibody specifically binds to mouse interleukin-2 (IL-2), a multifunctional cytokine that plays pivotal roles in immunity and tolerance. It is produced by activated T cells and affects the activation, growth, proliferation and/or differentiation of various cell types including T and B lymphocytes and their precursors, LAK cells, NK cells, and monocytes/macrophages. IL-2 mediates its biological activities by binding to IL-2 receptor complexes. The intermediate affinity IL-2R is comprised of IL-2Rβ (CD122) and common gamma chain (γc; CD132) subunits, whereas the high-affinity IL-2R is comprised of IL-2Rα (CD25), IL-2Rβ, and γc subunits. The JES6-5H4 monoclonal antibody binds to IL-2 and neutralizes its biological activity.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon™ BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.
566296 Rev. 1
Format Details
BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV421
407 nm
423 nm
566296 Rev.1
Citations & References
View product citations for antibody "566296" on CiteAb
Development References (4)
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Abrams J. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001; 1:6.20-6.21. (Clone-specific: ELISA). View Reference
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Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Clone-specific: ELISA, Immunoprecipitation). View Reference
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Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology: Flow cytometry). View Reference
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Sander B, Hoiden I, Andersson U, Moller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993; 166(2):201-214. (Clone-specific: ELISA). View Reference
566296 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.