The 217-170 monoclonal antibody specifically recognizes mouse IgD of Igh-C[b] haplotype (eg, C57BL/6, C57BL/10, SJL). It does not react with IgD of Igh-C[a] or related haplotypes (eg, BALB/c, AKR, CBA, C3H/He, C58, DBA/1, DBA/2, NZB, PL, RIII), or Igh-C[e] haplotype (eg, A). 217-170 antibody is effective for detection of cell-surface Ig by immunofluorescent staining with flow cytometric analysis. It has not been shown to stimulate B-cell proliferation.
The antibody was conjugated to BD Horizon™ BUV496 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 496-nm. BD Horizon BUV496 can be excited by the ultraviolet laser (355 nm) and detected with a 515/30 nm filter with a 450LP. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into the channel detecting BD Horizon V500 or BV510 (eg, 525/40-nm filter). However, the spillover can be corrected through compensation as with any other dye combination.