The M-A261 monoclonal antibody specifically binds to CD26. CD26 is also known as dipeptidyl peptidase IV (DPPIV/DPP IV, DPP4) or adenosine deaminase binding protein (ADABP). CD26 is a 110 kDa type II transmembrane glycoprotein. CD26 is expressed by T lymphocytes and increases upon activation. It is also expressed by B cells, NK cells, and macrophages. CD26 is an ectoenzyme which plays an integral role in T-cell proliferation and may interact with extracellular matrix proteins such as fibronectin or collagen.
The antibody was conjugated to BD Horizon™ BUV496 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 496-nm. BD Horizon BUV496 can be excited by the ultraviolet laser (355 nm) and detected with a 515/30 nm filter with a 450LP. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into the channel detecting BD Horizon V500 or BV510 (eg, 525/40-nm filter). However, the spillover can be corrected through compensation as with any other dye combination.