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Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
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The 5H10 monoclonal antibody specifically binds to mouse CD209a, or CIRE, which is the most closely related homolog of human DC-SIGN. In the mouse, several homologs of human CD209 (DC-SIGN) have been described. CD209a is a 238-amino acid type-II transmembrane C-type lectin, with an extracellular carbohydrate recognition domain. It can bind to mannosylated ligands in a Ca++ dependent manner. CD209a is expressed by some conventional CD4+ and CD4-CD8- dendritic cells (DC) but not by CD8+ DC. It is also expressed on monocytes, splenic plasmacytoid pre-DC, macrophages, and resident peritoneal cells. CD209a expression is downregulated upon cellular activation. CD209a is thought to play a role in the internalization and presentation of antigens to initiate immune responses.
Development References (7)
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Baribaud F, Pohlmann S, Sparwasser T, et al. Functional and antigenic characterization of human, rhesus macaque pigtailed macaque, and murine DC-SIGN. J Virol. 2001; 75(21):10281-10289. (Biology). View Reference
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Caminschi I, Corbett AJ, Zahra C, et al. Functional comparison of mouse CIRE/mouse DC-SIGN and human DC-SIGN. Int Immunol. 2006; 18(5):741-753. (Immunogen: Flow cytometry, Fluorescence activated cell sorting, Western blot). View Reference
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Caminschi I, Lucas KM, O'Keeffe MA, et al. Molecular cloning of a C-type lectin superfamily protein differentially expressed by CD8alpha(-) splenic dendritic cells. Mol Immunol. 2001; 38(5):365-373. (Biology). View Reference
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O'Keeffe M, Hochrein H, Vremec D, et al. Mouse plasmacytoid cells: long-lived cells, heterogeneous in surface phenotype and function, that differentiate into CD8(+) dendritic cells only after microbial stimulus. J Exp Med. 2002; 196(10):1307-1319. (Clone-specific: Flow cytometry). View Reference
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Parent SA, Zhang T, Chrebet G, et al. Molecular characterization of the murine SIGNR1 gene encoding a C-type lectin homologous to human DC-SIGN and DC-SIGNR. Gene. 2002; 293(1):33. (Biology). View Reference
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Park CG, Takahara K, Umemoto E, et al. Five mouse homologues of the human dendritic cell C-type lectin, DC-SIGN. Int Immunol. 2001; 13(10):1283-1290. (Biology). View Reference
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Ponichtera HE, Shainheit MG, Liu BC, et al. CD209a expression on dendritic cells is critical for the development of pathogenic Th17 cell responses in murine schistosomiasis. J Immunol. 2014; 192(10):4655-4665. (Clone-specific: Flow cytometry). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.