PE Mouse Anti-Human CD31

BD™ PE Mouse Anti-Human CD31

Name: PE Mouse Anti-Human CD31
Brand: BD™
SKU: 340297

Clone L133.1

(RUO (GMP))

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Product Details

Brand: BD™

Alternative Name: EndoCAM; GPIIA'; PECA1; PECAM1; Platelet endothelial cell adhesion molecule

Reactivity: Human

Isotype: Mouse IgG1, κ

Immunogen: Purified Human Natural Killer Cells

Application: Flow cytometry

Concentration: 12.5 μg/mL

Vol. Per Test: 20 μL

Workshop Number: V T-CD31.07

Entrez Gene ID: 5175

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: RUO (GMP)

Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

340297 Rev. 1

Antibody Details

L133.1

The CD31 (Anti-PECAM-1) antibody, clone L133.1, is derived from the fusion of Sp2/0 mouse cells with spleen cells from a BALB/c mouse immunized with purified human natural killer cells.

The CD31 (Anti-PECAM-1) antibody recognizes the platelet/endothelial cell adhesion molecule-1 (PECAM-1), a 130 to 140-kilodalton (kDa) single-chain integral membrane glycoprotein that is a member of the immunoglobulin gene superfamily. The CD31 antigen is composed of six extracellular immunoglobulin-like domains belonging to the C2 group. C2 domains are also found in other members of the immunoglobulin superfamily, the cell adhesion molecules (CAMs). The CD31 antigen functions as a vascular cell adhesion molecule and is involved in the process of leucocyte migration through the intercellular junctions of vascular endothelial cells. It may also be involved in thrombosis, angiogenesis, wound healing, and inflammation.

340297 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

340297 Rev.1

Citations & References

View product citations for antibody "340297" on CiteAb

Development References (13)

Albelda SM, Buck CA. Integrins and other cell adhesion molecules.. FASEB J. 1990; 4(11):2868-80. (Biology). View Reference
Albelda SM, Muller WA, Buck CA, Newman PJ. Molecular and cellular properties of PECAM-1 (endoCAM/CD31): A novel vascular cell–cell adhesion molecule. J Cell Biol. 1991; 114(5):1059-1068. (Biology).
Bordessoule D, Jones M, Gatter KC, Mason DY. Immunohistological patterns of myeloid antigens: tissue distribution of CD13, CD14, CD16, CD31, CD36, CD65, CD66 and CD67.. Br J Haematol. 1993; 83(3):370-83. (Biology). View Reference
Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
Clinical and Laboratory Standards Institute. 2005. (Biology).
Horak ER, Leek R, Klenk N, et al. Angiogenesis, assessed by platelet/endothelial cell adhesion molecule antibodies, as indicator of node metastases and survival in breast cancer.. Lancet. 1992; 340(8828):1120-4. (Biology). View Reference
Metzelaar MJ, Korteweg J, Sixma JJ, Nieuwenhuis HK. Comparison of platelet membrane markers for the detection of platelet activation in vitro and during platelet storage and cardiopulmonary bypass surgery.. J Lab Clin Med. 1993; 121(4):579-87. (Biology). View Reference
Miettinen M. Immunohistochemistry in tumor diagnosis. Ann Med. 1993; 25(3):221-233. (Biology).
Muller WA, Weigl SA, Deng X, Phillips DM. PECAM-1 is required for transendothelial migration of leukocytes. J Exp Med. 1993; 178(2):449-460. (Biology). View Reference
Newman PJ, Berndt MC, Gorski J, et al. PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily.. Science. 1990; 247(4947):1219-22. (Biology). View Reference
Simmons DL, Walker C, Power C, Pigott R. Molecular cloning of CD31, a putative intercellular adhesion molecule closely related to carcinoembryonic antigen. J Exp Med. 1990 June; 171(6):2147-2152. (Biology). View Reference
Stockinger H, Gadd SJ, Eher R, et al. Molecular characterization and functional analysis of the leukocyte surface protein CD31. J Immunol. 1990 December; 145(11):3889-3897. (Biology). View Reference
Tawia SA, Beaton LA, Rogers PA. Immunolocalization of the cellular adhesion molecules, intercellular adhesion molecule-1 (ICAM-1) and platelet endothelial cell adhesion molecule (PECAM), in human endometrium throughout the menstrual cycle.. Hum Reprod. 1993; 8(2):175-81. (Biology). View Reference

View All (13)

340297 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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