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APC Mouse Anti-Human CD95
Product Details
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Mouse C3H, also known as C3H/He, C3H/Bi IgG1, κ
Human CD95-transfected L Cells
Flow cytometry
50 μg/mL
5 μL
VI C-64
Phosphate buffered saline with gelatin and 0.1% sodium azide.

Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

340481 Rev. 1
Antibody Details
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The CD95, clone DX2, is derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from C3H/He mice immunized with L cells transfected with CD95 antigen.

The CD95 recognizes a 48-kdalton (kDa) antigen (Fas/APO-1) that is a member of the tumor necrosis factor/nerve growth factor (TNF/NGF) superfamily. It is associated with activated cells. Cross linking of CD95 antigen on activated cells can elicit apoptosis.

340481 Rev. 1
Format Details
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Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Red 627-640 nm
651 nm
660 nm
340481 Rev.1
Citations & References
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View product citations for antibody "340481" on CiteAb

Development References (11)

  1. Amasaki Y, Kobayashi S, Takeda T, et al. Up-regulated expression of Fas antigen (CD95) by peripheral naive and memory T cell subsets in patients with systemic lupus erythematosus (SLE): A possible mechanism for lymphopenia. Clin Exptl Immunol. 1995; 99:245-250. (Biology).
  2. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  3. Cifone MG, De Maria R, Roncaioli P, et al. Apoptotic signaling through CD95 (Fas/Apo-1) activates an acidic sphingomyelinase. J Exp Med. 1994; 180(4):1547-1552. (Biology). View Reference
  4. McClosky TW, Oyaizu N, Pahwa S. Review: Apoptosis, HIV infection, and the Fas antigen. Clin Immunol Newsletter. 1995; 15:105-113. (Biology).
  5. Miyawaki T, Uehara T, Nibu R, et al. Differential expression of apoptosisrelated Fas antigen on lymphocyte subpopulations in human peripheral blood. J Immunol. 1992; 149:3753-3758. (Biology).
  6. Möller P, Koretz K, Leithäuser F, et al. Expression of APO-1 (CD95), a member of the NFG/TNF superfamily, in normal and neoplastic colon epithelium. Int J Cancer. 1994; 57:371-377. (Biology).
  7. Oehm A, Behrmann I, Falk W, Pawlita M, et al. Purification and molecular cloning of the APO-1 cell surface antigen, a member of the tumor necrosis factor/nerve growth factor receptor superfamily. J Biol Chem. 1992; 140:197-205. (Biology).
  8. Owen-Schaub L, Angelo L, Radinsky R, Ware C, et al Soluble Fas/APO-1 in tumor cells: A potential regulator of apoptosis? Cancer Letters. 1992; 140:197-205. (Biology).
  9. Owen-Schaub LB, Yonehara S, Crump III WL, Grimm EA. DNA fragmentation and cell death is selectively triggered in activated human lymphocytes by Fas antigen engagement. Cell Immunol. 1992; 140:197-205. (Biology).
  10. Robertson MJ, Ritz J. Knapp W, Dörken B, Gilks W, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:1142-3.
  11. Shima Y, Nishimoto N, Ogata A, Fujii Y, Yoshizaki K, Kishimoto T. Myeloma cells express Fas antigen/APO-1 (CD95) but only some are sensitive to anti-Fas antibody resulting in apoptosis. Blood. 1995; 85:757-764. (Biology).
View All (11) View Less
340481 Rev. 1

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