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Anti-Human CD79b FITC/Lambda PE/CD19 PerCP-Cy™5.5

BD Oncomark™ Anti-Human CD79b FITC/Lambda PE/CD19 PerCP-Cy™5.5

(RUO (GMP))
Product Details
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BD Oncomark™
Human
Flow cytometry
RUO (GMP)
Phosphate buffered saline with BSA and 0.1% sodium azide.


Description

The CD79b antibody, clone SN8 (3A2-2E7-1F5), is generated from the fusion of NS-1 myeloma cells with spleen cells from BALB/c mice immunized with an antigen prepared from the membrane of B-prolymphocytic leukemia (B-PLL) cells.

The Anti-Lambda antibody, clone 1-155-2, is derived from hybridization of P3-X63- Ag8.653 mouse myeloma cells with cells from BALB C/J mice immunized with human IgA1-λ myeloma protein.

The CD19 antibody, clone SJ25C1, is derived from the hybridization of Sp2/0 mouse cells with spleen cells from BALB/c mice immunized with NALM1 + NALM16 cells.

The CD79b antibody reacts with the CD79b protein and the epitope lies on the extra domain of the peptide. CD79b is helpful in delineating signal transduction pathway activated via antibody receptors during different stages of B-cell differentiation.

The Anti-Lambda antibody is specific for lambda light chains of human immunoglobulins.

The CD19 antibody recognizes a 90-kilodalton (kDa) antigen that is present on human B lymphocytes.

Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

335801 Rev. 1
Components
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Description Clone Isotype EntrezGene ID
Ig, λ Light Chain PE 1-155-2 IgG1, λ 3535
CD19 PerCP-Cy5.5 SJ25C1 IgG1, κ 930
CD79B FITC 3A2-2E7 IgG1, κ 974
335801 Rev. 1
Citations & References
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Development References (21)

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  3. Clinical and Laboratory Standards Institute. 2005. (Biology).
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  6. Garcia Vela JA, Delgado I, Benito L, et al. CD79b expression in B cell chronic lymphocytic leukemia: its implication for minimal residual disease detection. Leukemia. 1999; 13:1501-1505. (Biology).
  7. Geary WA, Frierson HF, Innes DJ, Normansell DE. Quantitative criteria for clonality in the diagnosis of B-cell non-Hodgkin's lymphoma by flow cytometry. Mod Pathol. 1993; 6:155-161. (Biology).
  8. Johnson A, Cavallin-Stahl E, Akerman M. Flow cytometric light chain analysis of peripheral blood lymphocytes in patients with non-Hodgkin's lymphoma. Br J Cancer. 1985; 52(2):159-168. (Biology). View Reference
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  10. Letwin BW, Wallace PK, Muirhead KA, Hensler GL, Kashatus WH, Horan PK. An improved clonal excess assay using flow cytometry and B-cell gating. Blood. 1990; 75:1178-1185. (Biology).
  11. Loken MR, Shah VO, Dattilio K, Civin C. Flow cytometric analysis of human bone marrow, I: Normal erythroid development. Blood. 1987; 69:255-263. (Biology).
  12. Moldenhauer G, Dörken B, Schwartz R, Pezzutto A, Knops J, Hammerling GJ. Analysis of ten B lymphocyte-specific workshop monoclonal antibodies. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human B Lymphocytes. New York: Springer-Verlag; 1986:61-67.
  13. Nadler LM. B Cell/Leukemia Panel Workshop: summary and comments. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human B Lymphocytes. New York: Springer-Verlag; 1986:3-43.
  14. Nakamura T, Kubagawa H, Cooper MD. Heterogeneity of immunoglobulin-associated molecules on human B cells identified by monoclonal antibodies. Proc Natl Acad Sci U S A. 1992; 89(18):8522-8526. (Biology). View Reference
  15. Okazaki M, Luo Y, Han T, Yoshida M, Seon BK. Three new monoclonal antibodies that define a unique antigen associated with prolymphocytic leukemia/non-Hodgkin's lymphoma and are effectively internalized after binding to the cell surface antigen. Blood. 1993; 81(1):84-94. (Biology). View Reference
  16. Rawstron AC, Kennedy B, Evans PAS, et al. Quantitation of minimal disease levels in chronic lymphocytic leukemia using a sensitive flow cytometric assay improves the prediction of outcome and can be used to optimize therapy. Blood. 2001; 98:29-35. (Biology).
  17. Reichert T, DeBruyere M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol. 1991; 60(2):190-208. (Biology). View Reference
  18. Sanchez M, Misulovin Z, Burkhardt AL, et al. Signal transduction by immunoglobulin is mediated through Ig α and Ig β. J Exp Med. 1993; 178:1049-1055. (Biology).
  19. Tedder TF, Zhou LJ, Engel P. The CD19/CD21 signal transduction complex of B lymphocytes. Immunol Today. 1994; 15(9):437-442. (Biology). View Reference
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  21. Zomas AP, Matutes E, Morilla R, Owusu-Ankomah K, Seon BK, Catovsky D. Expression of the immunoglobulin-associated protein B29 in B cell disorders with the monoclonal antibody SN8 (CD79b). Leukemia. 1996; 10(12):1966-1970. (Biology). View Reference
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335801 Rev. 1

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