Description
CD8, clone SK1, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood T lymphocytes. CD28, clone L293, is derived from hybridization of mouse Sp2/0-Ag14 myeloma cells with spleen cells from BALB/c mice immunized with the HPB-ALL T-cell line. The CD8 (Leu-2a) antigen is present on the human suppressor/cytotoxic T-lymphocyte subset as well as on a subset of natural killer (NK) lymphocytes. The CD8 antigenic determinant interacts with class I major histocompatibility complex (MHC) molecules resulting in increased adhesion between the CD8+ T lymphocytes and the target cells. Binding of the CD8 antigen to class I MHC molecules enhances the activation of resting T lymphocytes. CD8 recognizes an antigen expressed on the 32-kdalton (kDa) α-subunit of a disulfide-linked bimolecular complex. The cytoplasmic domain of the α-subunit of the CD8 antigen is associated with the protein tyrosine kinase p56lck. The CD28 antigen, a disulfide-linked homodimeric glycoprotein (Mr 44 kDa), is a cell adhesion molecule (CAM) and functions as the ligand for the B7/BB-1 antigen that is present on activated B lymphocytes. Interaction of the CD28 antigen with the B7/BB-1 antigen costimulates CD2 and CD3 antigen/T-cell antigen receptor (TCR)–dependent T-cell–mediated cytotoxicity.
Preparation And Storage
The Simultest reagent is supplied as a combination of CD8 FITC and CD28 PE in 1.0 mL of phosphate-buffered saline. Buffered saline contains gelatin and 0.1% sodium azide. The vial should be stored at 2° to 8°C and protected from prolonged exposure to light. Simultest reagents should not be frozen. Each Simultest reagent is stable for the period shown on the bottle label when stored as directed.
| Description | Quantity/Size | Part Number | Clone | Isotype |
|---|---|---|---|---|
| CD28 PE | N/A | 50-7313-0.1mg/mL | SK1 | IgG1, κ,IgG1, κ |
| N/A | N/A | 50-8047 | L293 | N/A |
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